Bio125 Lab 2

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The human eye cannot distinguish objects smaller than

0.1mm
Light microscope can see:
- plant cell
- animal cell
- bacterium
Electron microscope can see:
- bacterium
- virus
- small molecule
Returning the microscope: 6 steps
- Always leave the lowest power objective (4X) in place (short red one)
- Remove the slide
- Place the stage at its lowest level
- Turn off the main light switch off
- Turn the light intensity control knob to its lowest level
- Wrap the power cord properly
- Main light switch
- turns the microscope’s light source on/off
- Light intensity control knob
- adjusts the intensity of the microscope’s light source (“dimmer switch”)
- Slide holder
- holds the slide on the stage
- Stage
- where the slide is placed on the microscope
- Iris diaphragm knob
- regulates the among of light that passes from the light sources to the stage
- Stage movement knobs
- move the slide on the stage
- Coarse focus knobs
- knob used to bring object into appropriate focus; used only with scanning power objective
Red arrows
- Fine focus knob
- knob used to bring the object into appropriate focus, used with all objective lenses
- Objective lenses
- magnifies the slide
Scanning objective (red):

4x objective
Low power objective (yellow):

10x objective
High power objective (blue):

40x objective
Hi-dry objective (purple):

60x objective
Eyepieces (ocular lenses):

10x magnifying power
Interpupillary distance scale:

measure distance between eyepieces
Ocular lenses (eyepieces) have a magnifying power of

10X
Total magnification=

objective lens mag. X ocular mag.
The total magnification of a specimen underneath a microscope using the high power objective lens is…

Total magnification= (40)(10)= 400x
- Plasma membrane/ cell membrane
- Protects the cell and regulates what comes in and out of the cell
- Nucleus
- Control center of the cell/contains the DNA
- Nuclear envelope
- Regulates what comes in and out of the nucleus
- Nucleolus
- Produces the large and small ribosomal subunits
- Chromatin
- Genetic information
- Cytosol
- Suspends the cellular elements
Hint: "tiny blue dots"
- Ribosomes
- Site of translation (protein synthesis)
Hint: "tiny white dots"
- Ribosomes
- Site of translation (protein synthesis)
- Rough endoplasmic reticulum
- Produces membrane-bound proteins
- Smooth endoplasmic reticulum
- Ca2+ storage/lipid metabolism
- Golgi apparatus
- Packages and ships membrane-bound proteins
- Mitochondria
- Produces ATP
- Lysosomes
- Intracellular digestion
- Peroxisomes
- Detoxification
- Vesicles
- Intracellular transport
- Centrosome
- Involved in cell division
Hint: "striped"
- Centrioles
- Involved in cell division

Author

jocelyn8

360982

Card Set

Bio125 Lab 2

Description

Lab Exam 1

Updated

2023-03-08T05:17:45Z