what are the four genus under the family vibrionaceae?
Vibrio
Aeromonas
Plesiomonas
all vibrionaceae organisms have what oxidase result?
+
all vibrionaceae organisims move by what type of motility?
ploar flagellation
true or false
Vibrionaceae organisms do not produce gas
false
what does TCBS stand for?
tiosulfate-citrate-bile salts-sucrose
TCBS is used for what family of organisms?
vibrionaceae
Vibrionaceae organisms are what type of oxygen users?
facultative anaerobe
what is the cytochrome oxidase result for Vibrionaceae organisms?
+
what is the indole result for Vibrionaceae?
+
what is the result of dextrose fermentation for Vibrionaceae?
+
what is the habitat for Vibrio species?
sea water
brackish
what is the habitat for Aeromonas species?
fesh water
salt water
brackish water
what is the habitat for Plesiomonas species?
fresh water
this is the most common cause of gastroenteritis from contaminated water or seafood?
Vibrio cholerae
true or false
Vibrio cholerae can be transmitted by contact exposure with mucosa or open skin?
true
Rice water stool is associated with what type of cholera caused by V. cholerae?
asian cholera
what are the three signs and symptoms associated with asian cholera?
watery stools
mucus flecks
Cholera toxin causes hypersecretion in GI tract
what is the toxin in V. cholerae that causes hypersecretion in the GI tract?
cholera toxin (CT)
V. cholerae is: (Pick one)
(A) Haplophilic
(B) Haplophobic
(A) haplophilic
what is the organism that is divided into serogroups based on somatic "O" antigens?
V. cholerae
all epidemic strains of ___________ were assigned originally to serotype "O" subgroup 1 (O1).
V. cholerae
what is the appearance of V. cholerae on the TCBS?
yellow
the yellow appearance of V. cholerae on the TCBS is caused by what?
sucrose
what is the result for V. cholerae in O1 antisera?
+
what is the V. cholerae result for string test(0.5 bile salt)?
+
what is the reagent used in the string test?
0.5 bile salt
what is the V. cholerae for lactose and arginine?
=
what is the V. cholerae result for sucrose, lysine, and ornithine?
+
what is the test that differentiates classical and El Tor V. cholerae?
VP
Classical (=)
El Tor (+)
what are the two V. cholerae serotypes that produce cholera toxin?
O130
O1
what are two clinical significances of V. parahaemolyticus?
wound infections
septicemia
this Vibrio species can be contracted through contaminated seafood, raw fish, and shellfish.
V. parahaemolyticus
what is the treatment of V. parahaemolyticus?
rehydration
what is the TCBS result for V. parahaemolyticus?
(=) green
what is the O1 antisera result for V. parahaemolyticus?
=
which Vibrionaceae species can cause meningitis, pneumonia, conjunctivitis, and endocarditis?
Aeromonas hydrophila
humans and animals are carriers of what water loving bacteria?
A. hydrophilia
what are the three means of transmission of A. hydrophila?
ingestion
skin exposure
inoculation
A. hydrophila exhibits what type of hemolysis on a BAP?
beta hemolytic
what result on TSI differentiates A. hydrophila and P. shigelloides?
A. hydrophila: Gas(+)
P. shigelloides: Gas(=)
what is the lactose result on Aeromonas hydrophila?
=
what is the sucrose result on A. hydrophila?
+
what is LDC and ADC results on A. hydrophila?
+
what is the ornithine result for A. hydrophila?
=
what is the result of A. hydrophila on TCBS?
no growth
Plesiomonas shigelloides can cause what two disease states in immunocompromised patients and infants?
gastroenteritis
septicemia
what are the two means of transmission of Plesiomonas shigelloides?
ingestion of contaminated H2O
reptiles
what is the habitat of P. shgelloides?
Fresh warm water
P. shigelloides exhibits what type of hemolysis on a BAP?
non-hemolytic
what are the lactose and sucrose results of P. shigelloides?
=
what are the ldysine and arginine results of P. shigelloides?
+
what is the ornithine result for P. shigelloides?
+
what is the TCBS result for P. shigelloides?
=
Vibrionaceae have what type of appearance on gram stain?
Gram negative curved or straight rods
what is the test used to ID gram negative bacilli?
API NE
Laboratory isolation of this genus is different depending on the relative location and incidence of infection?
Vibrio
Where is there few positive cultures of Vibrio?
american midwest
in what areas should a tech incorporate TCBS?
endemic
true or false
routine use of TCBS is recommended in endemic areas
false
what should be used for laboratory isolation of Plesiomonas and Aeromonas?
routine enteric media
if Vibrio is suspected what three medias should be used?
BAP
MAC
TCBS
what media is used for isolation and enrichment of Vibrio?
alkaline peptone water (pH 8.4)
what is the pH for alkaline peptone water?
8.4
what media should be used when subculturing from alkaline peptone water?
TCBS agar
how do sucrose fermenters appear on the TCBS?
yellow
what are the pH indicators in the TCBS?
bromthymol blue
thymol blue
what is the pH of TCBS?
8.6
what is the media that is selective for Vibrio?
TCBS
BAP can be made selectivew for Aeromonas with the addition of what?
ampicillin
this can improve recovery of Aeromonas from stool.
ampicillin
what are the two medias that have a high recovery rate for Aeromonas?
MAC
XLD
this organism can be recovered using Sheep Blood Agar without ampicillin or enteric media.
Plesiomonas
what should be used fo there is a delay in plating Vibrio?
Cary-Blair semi solid transport media
Vibrio spp is sensitve to what three things?
Drying
exposure to sunlight
Acid pH
APW should be used for _______ numbers of vibrio.
small
true or false
BAP is adequate for growth of vibrio spp.
true
true or false
TCBS can be inoculated heavily
true
_______ grows well on BHI and BAP even without added salt.
Vibrio
what is the test used to differntiate Vibrionaceae from Enterobacteriaceae.
oxidase
what test is used to differentiate pseudomonas from aeromonas?
Indole
Aeromonas (+)
What five tests differentiate Aeromonas from plesiomonas spp?
Aeromonas:
beta hemolytic
DNAse (+)
VP (+)
ODC (=)
Mannitol (+)
what is the catalase result for all Pasteurellaceae species?
+
in pasteurellaceae acid is produced by utilizing what three sugars?
glucose
fructose
sucrose
what is the starch or salicin hydrolysis result for Pasteurellaceae?
=
Pasteurellaceae organisms are susceptible to what three antibiotics?
penicillin
cephalosporin
tetracyclines
true or false
Pasteurellaceae does not reduce nitrate to nitrite
false
Pasteurellaceae organisms exhibit what type of motility?
non motile
what is the appearance of Pasteurellaceae on a gram stain?
Gram negative coccobacili or rods
Pasteurellaceae may stain bipolar in what two stains?
wright
giemsa
what family of gram negative rods resembles a closed safety pin?
Pasteurellaceae
pasteurellaceae organisms are what type of oxygen users?
facultative anaerobe
most Pasteurellaceae organisms will not grow on what media?
MAC
Pasturella multocida is mostly isolade from _______ exposure.
animal
what organism is commonly isolated from cat bites or scratches, dog bites, or other domestic or wild animals.
Pasteurella multocida
what are the four clinical significances of P. multocida?
soft tissue infections
Bacteremia
Respiratory tract infections
Infections due to past and current animal exposure
this organism grows well on BAP and CHOC but does not grow on the MAC.
P. multocida
P. multocida is inhibited by what class of media?
enteric
this is a translucent, smooth convex, and butyraceous gamma hemolytic organism that is 1-2mm and has a disticnt musty or mushroom odor.
P. multocida
this organism is normal vaginal flora.
Gardnerella vaginalis
what are the three clinical significances of G. vaginalis?
non-specific vaginosis
Bacteremia including neonates
postpartum endometriosis
This organism appears as gramn variable pleomorphic rods.
G. vaginalis
G. vaginalis exhibits what type of motility?
non-motile
these are diagnostic of G. vaginalis.
Clue cells
these are epithelial cells covered by small gram negative rods.
Clue cells
what is the odor of G. vaginalis with KOH?
amine
which organism was formerly under genus Haemophilus but now has no specific taxonomic grouping?
G. vaginalis
G. vaginalis forms what size colonies on BAP?
punctate
G. vaginalis grows well on what media?
CHOC
G. vaginalis exhibits what type of hemolysis on the HBIT agar (Human Blood)?
beta hemolytic
how should G. vaginalis be incubated?
35oC for 48hrs in 5% CO2
what is the result for hippurate and starch hydrolysis on G. vaginalis?
+
what are the five confirmatory biochemical testings and their results for P. multocida?
Lactose: =
Maltose: =
TSI A/A: no gas
Urease: =
ODC: +
commonly isolated pathogenic campylobacter species have what catalase result?
+
what is the only Camplyobacter organism that yields a + hippurate hydrolysis test?
C. jejuni
what is the incubation temperature for C. fetus?
25oC
what are the incubation temperatures for C. jejuni and C. coli?
42oC
what two organisms are susceptible to Naladixic acid (30ug)?
C. jejuni
C. coli
what two organisms are resistant to Naladixic acid (30ug)?
C. fetus
C. lari
what is the quantity of naladixic acid used for campylobacter susceptibility testing?
30ug
what are the three organisms that are resistant to Cephalotin (30ug)?
C. jejuni
C. coli
C. lari
what is the organism that is susceptible to Cephalotin (30ug)?
C. fetus
what quantity of Cephalotin used for Campylobacter testing?
30ug
what genus of organisms has confluent growth around streak line?
campylobacter
what is the pale staining gram negative organism that needs extended safranin counterstain time?
Campylobacter
for campylobacter safranin counterstain time should be extened to how many minutes?
10 minutes
how much basic fuchsin should be added per liter of safranin for the staining of campylobacter?
1/2g
this is a pleomorphic, slender, spirally curved rod that forms an 'S', long spirals, or classic gull wing shape?
Campylobacter
what type of oxygen user is campylobacter?
obigate microaerophilic
what is the incubation atmosphere for campylobacter?
5% O2
10% CO2
85% Nitrogen
for what organism is increased CO2 not recomended, and gas pouches are commercially available?
Campylobacter
Campylobacter exhibits what type of motility in phase contrast or dark field microscopy?
darting/tumbling motility
Campylobacter exhibits darting/tumbling motility in what type of microscopy?
phase contrast
dark field
the number of cases of gastroenteritis due to what organism surpassed salmonella and shigella.
C. jejuni
what are four signs and symptoms of C. jejuni gastroenteritis?
crampy abdominal pain
fever
chills
bloody diarrhea
many patients with Guillain-Barre syndrome (GBS) are infected with this bacteria prior to onset of neurological symptoms.
C. jejuni
C. jejuni may cause occasional _________.
bacteremia
what ubiquitous organism can be contracted from raw milk, partially cooked poultry, or contaminated water?
C. jejuni
C. jejuni infections are self-limiting and reslove in _____ days.
3-7
whar oraly antibiotic is perscribed for severe C. jejuni cases?
erythromycin
this is an infrequent human pathogen that is seen in systemic illness in patients with chronic hepatic, renal, or neoplastic disease.
C. fetus
procitis and proctocolitis in homoseual men is seen in what bacteria?
C. fetus
what organism is seen in premature labor, neonatal sepsis, spetic abortion, and heart valve endocarditis?
C. fetus
what is the organism that is closely related to C. jejuni and causes diarrhea?
C. coli
C. coli accounts for what percent of campylobacter enteritis in humans?
5-10%
which campylobacter species cause UTIs very rarely?
C. coli
if Hippurate hydrolysis test is not performed how are Campylobacter reported?
C. jejuni/C. coli
Definitive Campylobacter species ID depends on determination of ________ characteristics.
phenotypic
what are the two selective medias used for Campylobacter?
Campy-BAP
Campy-Thio
true or false
CO2 incubator is not recommended for Campylobacter species
true
What is the best medium for Campylobacter?
Charcoal-Based Selective medium (CSM)
high temperature suppresses growth of what in campylobacter?
normal competing flora
at what intervals should Campylobacter be examined?
24
48
72hrs
what may grow as break through organisms and be confused with campylobacter?
Pseudomonas aeruginosa
this organism produces pyocyanin pigment and fruit-juice odor.
P. aeruginosa
what is the factor that includes hemin found in Hgb?
X factor
what is the factor that contains Nicotinamide adenine dinucleotide (NAD; coenzyme I)?
V factor
what is the identification test that bypasses problems with X factor test for Haemophilus?
ALA Porphyrin
what is the easiest technique for serotyping Haemophilus?
slide agglutination
what is the most common, most virulent serotype for Haemophilus?
Serotype b
what ar ethe recovered serotypes for Haemophilus?
A
c
d
e
f
biochemical methods of Haemophilus ID is based on acid from what five carbohydrate utilizations?
Sucrose
Fructose
Ribose
Xylose
Mannose
these are nonmotile, small, pale staining GNR or coccobacilli that are occasionally pleomorphic slender filaments seen on direct smears.
Haemophilus
what is the fastidious organism who's name means blood loving?
Haemophilus
what bacteria requires a vaccine for children due to neonatal sepsis with meningitis?
Haemophilus influenzae
what strain of Haemophilus is encapsulated?
H. influenzae
what is the encapsulated bacteria that causes epiglottitis, cellulitis with bacteremia, septic arthritis, and pneumonia?
H influenzae
Non encapsulated strains of H. influenzae will cause what four conditions?
Respiratory infections
Otitis media
Sinusitis
Bronchitis
what is the biogroup of H. influenzae that causes purulent conjunctivitis and brazilian purpuric fever?
aegyptius
when were ampicillin resistant strains of H. influenzae confirmed?
1974
H influenzae is resistant to what antibiotic?
ampicillin
what percent of H. influenzae produce Beta-lactamase?
20%
what are the following results for H. influenzae?
X factor
V factor
lactose
mannose
X factor: +
V factor: +
Lactose: =
Mannose: =
this organism is less common than H. influenzae but causes a wide variety of infections.
H. parainfluenzae
what are the following results for H. parainfluenzae?
X factor
V factor
Lactose
Mannose
X factor: =
V factor: +
Lactose: =
Mannose: =
what is the sexually transmitted bacteria that causes a painful ulcerative genital lesion known as a chancroid, enlarged inguinal lymph nodes and satellite infection?
H. ducreyi
what are the two specimens that H. ducreyi is isolated from?
swab from genital ulcers
aspirates from buboes
on which Haemophilus organism will you perform a direct gram stain smear?
H. ducreyi
what is the appearance of H. ducreyi on a gram stain?
school-of-fish
Railroad tracks
what are the following results for H. ducreyi?
X factor
V factor
Lactose
Mannose
X factor: +
V factor: =
Lactose: =
Mannose: =
this bacteria is a member of the HACEK family and is a rare cause of slow, progressive, subacute endocarditits.
H. aphrophilus
what are the following results for H. aphrophilus?
X factor
V factor
Lactose
Mannose
X factor: =
V factor: =
Lactose: +
Mannose: +
what bacteria can causes host tissue damage such as peptic ulcer disease, gastritis, and high risk of gastric carcinoma?
Helicobacter pylori
what are the following results of Helicobacter pylori?
H/H:
Catalase:
Urease:
H/H: (=)
Catalase: +
Urease: +
what is the biochemical identification of Haemophils based on?
growth requrements for X and V factors
what is the media used for initial cultivation of Haemophilus?
Chocolate agar
what is the temperature used to lyse sheeps RBC's by gentle heat?
80oC
what genus of GNR has no hemolytic determination?
Haemophilus
TSA and MH are used for which genus of facultative GNRs?
Haemophilus
what genus of facultative GNR should be suspended in saline to reduce carryover of growth factors when mat streaking to TSA or MH?
Haemophilus
how far apart should X and V factor disks be placed on Haemophilus?
1 to 2 cm apart
how should Haemophilus be incubated?
35oC
3-5% ambient air
18-24 hours
what two organisms are differentiated from other organisms based on growth on chocolate or blood and gram stain morphology?
Haemophilus
Pasteurella
pasteurella and Haemophilus will not grow on what media?
MAC
Pasteurella nd Haemophilus both grow well on what media?
CHOC
Author
corbin19
ID
36074
Card Set
Facultative GNRs other than Enterobacteriaceae 7.7
Description
Microbiology unit 7.7, Facultative Gram Negative Rods other than Enterobacteriaceae