Activity 12: Spectrophotometric Determination of Protein in Eggwhite

It is used to measure light absorption or the amount of chemicals in a solution.

Why does an object appear colored?

What light energy does a solution absorb?

What can be used to determine the molecules in a soluton

It measures the absorption of light of a particular wavelength and energy by a solution.

What is the symbol of the intensity of light emerging from a solution?

What is the symbol of the intensity of light entering the solution?

It is the ratio of the intensity of light emerging from a solution to the intensity of light entering the solution.

What is the formula for transmittance?

These are the negative logarithms of transmittance.

What is the formula for absorbance?

The absorbance is proportional to:

What is the equation of the Beer-Lambert Law?

What is usually the basis in which the solution has its maximum absorbance?

From the Beer-Lambert Law, a plot of absorbance against concentration will yield a?

These are accurately known concentrations that the solution used to construct the line

It enables the absorbance of a substance in solution to be calibrated against its concentration.

The concentration of a substance in solution can be determined from the?

What is the standard curve if the concentration is low?

What is the standard curve if the concentration is high?

It is one of the many methods of protein determination and is used as a general test for peptide chains of at least three amino acids.

What is the positive reaction in a biuret reaction?

Why does a positive reaction in a biuret reaction yield a purple color?

What gives a positive biuret reaction?

Protein can be precipitated with _______ when interfering substances are present in a biuret reaction.

It carries a negative charge which neutralizes positive charges on proteins to form an insoluble protein.