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What does the electron microscope use?
Uses beam of electrons (e-), a number of electromagnetic lenses (that focus and diverge the beam of e-), a piece of photographic film and a specimen.
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What magnification can the electron microscope produces to?
Produces magnifications of up to 1,000,000 X.
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What are 2 types of electron microscopes?
- Transmitting Electron Microscope (TEM)
- Scanning Electron Microscope (SEM)
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What does the transmitting electron microscope do?
- Sends electrons through objects and reveals the mist detail.
- The TEM uses electromagnets as lenses to focus and magnify the image by bending the paths of the electrons.
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What does the transmitting electron microscope send through objects and why?
Sends electrons through objects and reveals the most detail.
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What does the scanning electron microscope do?
Photographs reflected electrons from surfaces and reveals 3-D structures.
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In the scanning electron microscope, what is the surface usually coated with?
The surface is usually coated with a thin film of gold.
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What are 7 differences between the light and electron microscopes?
- L: Uses light rays
- E: Uses beams of electrons.
- L: Focuses them with >= 2 convex lenses to illuminate an object.
- E: Focuses them with electromagnets to illuminate an object.
- L: Magnifies up to 1400 X.
- E: Magnifies up to 500,000 X.
- L: Low resolution (up to 200nm).
- E: High resolution (up to 1nm), (Beam of electrons has much smaller wavelength than light).
- L: Reveals nucleus, cell organelles, cell walls, vacuoles, chromatin.
- E: Reveals details of cell organelles and cell structures (such as cilia, flagella and membranes).
- L: Portable, relatively inexpensive
- E: Not portable, very expensive.
- L: Can examine living tissue (thin).
- E: Objects dead (in a vacuum).
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What’s the difference between the electron and light microscope in what they use and how they focus?
L: Uses light rays and focuses them with >=2 convex lenses to illuminate an object.
E: Uses beam of electrons and focuses them with electromagnets to illuminate an object.
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What’s the magnification difference between the 2 microscopes?
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What’s the resolution difference between the 2 microscopes?
- L: Low resolution (up to 200 nm).
- E: High resolution (up to 1nm), (Beam of electrons has much smaller wavelength than light).
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Why has the electron microscope have a higher resolution?
Beam of electrons has much smaller wavelengths than light.
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What do the 2 types of microscope reveal different?
- L: Reveals nucleus, cell organelles, cell walls, vacuoles, chromatin.
- E: Reveals details of cell organelles and cell structures (such as cilia, flagella, membranes).
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Which of the 2 microscopes are portable and relatively expensive?
Light microscope
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Which of the 2 microscopes can examine living tissue and why can’t the other?
Light can examine living tissue (thin) but the electron examines dead objects (in a vacuum).
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