The Pap test for cervical cancer involves microscopic examination of cervical cells for cancerous cells. A new, rapid diagnostic test to detect human papilloma virus (HPV) DNA before cancer develops is done without microscopic exam. The steps involved in this FastHPV test are listed. What is the second step?
D.
You have a small gene that you wish replicated by PCR. After 3 replication cycles, how many double-stranded DNA molecules do you have?
B.
Which of the following processes is NOT involved in making cDNA?
D.
A restriction fragment is..
C.
A source of heat-stable DNA polymerase is..
B.
PCR can be used to amplify DNA in a clinical sample. The following steps are used in PCR. What is the fourth step?
B.
If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is
C.
The reaction catalyzed by reverse transcriptase
E.
The following steps must be performed to make a bacterium produce human protein X:
1-Translation; 2-Restriction enzyme; 3-Prokaryotic transcription; 4-DNA ligase; 5-Transformation; 6-Eukaryotic transcription; 7-Reverse transcription.
Put the steps in the correct sequence.
B.
A technique used to identify bacteria carrying a specific gene is
D.
Large numbers of bacterial cells are NOT found in crown galls because
B.
You want to determine whether a person has a certain mutant gene. The process involves using a primer and Taq. This process is
B.
Which of the following can be used to make recombinant DNA?
C.
Foreign DNA can be inserted into cells by all of the following except
E.
PCR can be used to identify an unknown bacterium because
B.
Self-replicating DNA used to transmit a gene from one organism to another is a
B.
Subunit vaccines can be made by genetic modification of yeast cells. A side effect of these vaccines might be
E.
The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes