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What are the 3 main parts of a cell?
Plasma membrane, cytoplasm, and nucleus
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What is the general function of plasma membrane
It's is composed of lipid (phospholipid bilayer, cholesterol, and glycolipids) fluid serves as a barrier for the components of the cell to its outside environment
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What is the general function of cytoplasm
Everything found inside th plasma membrane. Composed of Cytosol (fluid in cell) and organelles. Has machinery for metabolism
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What is the general function of the nucleus
Organelle that is the control center of the cell containing chromosomes (genetic info.). Involved in protein synthesis and passage of genetic info to offspring.
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What is the difference between cytoplasm and cytosol?
Cytosol is the fluid in the cell ONLY. Cytoplasm is the Cytosol plus other organelles
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What the difference between ECF and ICF
The fluid that bathes the cells, found outside the cells. While ICF (Cytosol) is the fluid found inside the cells. Icf (pH=7) is slightly more acidic than ECF (pH=7.4)
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Describe the components of a plasma membrane
- Phospholipids form a bilayer. About 75% of the membrane. Phosphate head and fatty acid tail.
- Glycolipids- line surface of membrane. 5%. Acts as name tags to distinguish cells from invaders.
- And cholesterol. Wedged between fatty acid tail. 20%. Gives stiffness to membrane
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How are carbohydrates distributed in a plasma membrane and explain functions.
They are located on the outside of the membrane and are attached to glycolipids. Act as a name tag to defend against invaders.
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How are proteins (interval and peripheral) distributed in plasma membrane and functions of.
- Interval proteins go completely through the bilayer. Peripheral only adhere to one side of the membrane.
- The membrane proteins act as receptors to attach chemicals, channels to allow passage of diluted through plasma membrane, and carriers to carry diluted across plasma membrane (carriers that require ATP are called pumps Na/K pumps)
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What are the types of cellular differentiation
- Totipotent-stem cell that can become anything
- Pluripotent- cell can become any human tissue cell
- Multi potent- can differentiate into either cell lineage
- Oligopotent- can become one of a few different types.
- Unipotent- can only make more of its own type
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What are desmosomes and their importance in the body
Filaments that keep cells anchored down and together.
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What are gap junctions and their importance in the human body
Opening that let ions pass 1 cell to the next. Gives rhythm to cells and allows for rapid communication. Heart cells
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What are tight junction and their importance in the human body?
Keeps water that's supposed to be in the body, in. And water that's supposed to be outside, out. Keeps cells close, skin and stomach cells. Makes cells waterproof
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Describe diffusion
Movement of particles thru semi permeable membrane from higher to lower concentration without energy
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Describe facilitated diffusion
same as diffusion EXCEPT the movement is thru a protein carrier. No energy
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Describe filtration
same as diffusion BUT due to hydrostatic pressure it pushes things out. No atp
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Describe osmosis
Simple diffusion of water. No atp
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Describe active transport
Movement of particles thru semipermeable from low to high with the use of atp. Against concentration gradient
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Name 4 types of passive transport
Simple diffusion, facilitated diffusion, filtration, and osmosis
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Name 4 types of active transport
Exocytosis, endocytosis, phagocytosis, pinocytosis
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Describe exocytosis
Moving absolute out of a cell. Spits out wastes. Needs atp
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Describe endocytosis
Moving a solute into a cell by engulfing it in a vesicle. Phago and pinocytosis. Needs atp
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Describe phagocytosis
Cell eating. White blood cells do this
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Describe pinocytosis
Cell drinking. Small amounts.
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What are the effects of isotonic conditions on cells
A solution where the concentration is the same as ICF.
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Define and describe effects of hypertonic conditions of cells
Has higher concentration of solutes like protein or sodium on outside and low concentration of solvents (water) on inside compared to ICF. Cells lose water and shrivel.
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Define and describe effects of hypotonic solutions on cells
Has lower concentration of solutes (protein, sodium) outside cell and higher concentration of solvent (water) inside compared to ICF. Cells gain water and swell, may burst
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What is an organelle
Subcellular structure in Cytosol that carries out specialized metabolic functions
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Indentify and describe structure and function of nucleus
Control center, determines structure and function.
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Indentify and describe structure and function of ER and RER
- Membranous network of interconnected channels within cytoplasm.
- Rough ER - dotted with ribosomes and is responsible for protein synthesis
- Smooth ER- lacks ribosomes. Has more interconnected channels than RER. Responsible for synthesis of lipids (steroids), stores Ca++, metabolizes some carbs, and breaks down certain toxins
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Indentify and describe structure and function of Golgi apparatus
Sorts, modifies, and ships products (proteins) from rough ER. makes lysosomes
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Indentify and describe structure and function of lysosomes
Contains enzymes to break down and digest.
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Indentify and describe structure and function of peroxisome
Formed by ER. Holds enzymes that break down substances like very long chain fatty acids or amino acids.
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Indentify and describe structure and function of mitochondrion
Kidney bean shape. Powerhouse of cell, produces atp. Has own DNA and reproduces on its own
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Indentify and describe structure and function of ribosomes
Responsible for protein synthesis. Read coded messages from mRNA to assemble amino acids and polypeptides (protein)
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Indentify and describe structure and function of centrioles
Non membranous. Arranged in 9 groups of 3 on periphery. Their role is in cell division by forming the mitotic spindle and guiding chromatids and to opposite poles of the cell
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Describe the events that take place and their functional significance in stages of interphase up until mitosis
- Interphase- 3 phases (75% of the process) where cell is not dividing.
- G1- gap where growth and metabolic rates are established
- Synthesis- period of DNA replication
- G2- growth and prep for mitosis
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Describe events that take place and their functional significance in the stages of mitosis
- Mitosis- splitting nucleus into two, immediately after is cytokinesis.
- Prophase- sister chromatids become visible
- Metaphase- sister chromatids line up and are ready to split
- Anaphase- "GO" chromatids are pulled apart
- Telophase- 2 nuclei form around chromosomes
- Cleavage furrow- 2 cells are formed
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What is the difference between mitosis and cytokinesis
Mitosis is the division of the nucleus (the DNA) and cytokinesis is the separation of the cytoplasm.
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Describe DNA replication
- Happens during synthesis.
- Replication- entire strand is replicated. Scanned for mistakes
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Describe chromatin, chromosomes, and chromatid
- Chromatin- loosely organized DNA in nucleus of non dividing cell.
- Chromosomes- super organized DNA in cell preparing for mitosis
- Chromatid- two sister halves, split at centromere to form separate chromosomes in anaphase
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Give examples of cell types that divide by mitosis and examples of when mitosis is required
Most cells go thru mitosis. Necessary for fetus growth, tissue growth, and replacement of damaged tissue. Skin cells are constantly undergoing it to flake off surface and replace
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What is the difference in the type of reproduction between mitosis and meiosis
Mitosis is asexual, meiosis is sexual
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What is the difference in the location of mitosis and meiosis
Mitosis happens all throughout the body. Meiosis only happens in the gonads
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What is the difference in the purpose of mitosis and meiosis
Mitosis supplements healing and growth. Meiosis produces offspring and can vary
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What is the difference in the # of cells produced between mitosis and meiosis
Mitosis produces two identical daughter cells. Meiosis produces four daughter cells not identical
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What is the difference between the # of chromosomes in the daughter cells of mitosis and meiosis
Mitosis daughter cell has 46 chromosomes. Meiosis daughter cell has 23
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Define genetic code for protein syntheis
A system that enables 4 nucleotides (A,G,C,T,or U) to code for the amino acid sequences of all proteins
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Define gene for protein syntheis
A sequence of DNA that codes for one protein
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Define base triplet for protein synthesis
3 base sequence on DNA that codes for one protein
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Define codon for protein synthesis
3 base sequence on mRNA that codes one amino acid. 64 possible
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Define anticodon for protein syntheis
3 base sequence on tRNA that codes one amino acid. Corresponds to codons
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What is transcription
Process of copying genetic instructions from DNA to mRNA. Happens in nucleus. mRNA provides code for translation
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What is translation
Process of reading mRNA to form polypeptide (protein). Happens in cytoplasm on any ribosomes. tRNA brings correct amino acids to ribosomes for protein assembly.
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Explain the synthesis of mRNA
During transcription in the nucleus, the info from DNA is copied onto mRNA based on law of complimentary base pairing. This RNA can travel to ribosomes and synthesize protein now.
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What is the law of complimentary base pairing in RNA?
- Adenine to uracil
- Cytosine to guanine
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Explain the assembly of polypeptides
Ribosomes read codons on mRNA as it passes thru two subunits of ribosomes. tRNA brings amino acids from cytoplasm
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Ex. The codons of mRNA are UGA CCG GAU. Based of law of complimentary pairing, what is the anti codon
ACU GGC CUA
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What is the role of tRNA in protein synthesis
Transfer RNA. delivers amino acids to ribosomes so they can be assembled into a polypeptide chain
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What is the role of mRNA in protein syntheis
Messenger RNA- a Cody of the DNA that carries genetic information from DNA to ribosome from nucleus to cytoplasm
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What is the role of rRNA in protein syntheis
Ribosomal RNA. Makes the ribosomes themselves and helps "read" information form mRNA during translation
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What is the role of DNA in protein synthesis
It carries all the genetic information and is a code for protein synthesis. Determines what proteins are made.
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Explain how cells maintain homeostasis in terms of protein synthesis
Cells scan DNA for mutations. If there was an error it may cause cancer or a defect. Cells try to not let this happen
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