Lipid anchors

  1. Myristoylation Target, Linkage, Enzyme, & Motif
    • Target: N terminal only
    • Linkage: amide
    • Enzyme: Myristoyl transferase
    • Motif: H2N - M G X X X S...COO-

    (See slide #18)
  2. Myristoyl & Palmistoyl transferase uses __ & the target protein as a substrate
    myristoyl-CoA & palmistoyl-CoA
  3. Myristoylated protein processing
    Methionine is clipped off by protease, exposing Glycine. The myristoyl-CoA bond is broken.

    Note: No processing is required for Palmitoylation

    (See slide #18)
  4. Palmitoylation Linkage, Enzyme, & Motif
    • Target: not specific
    • Linkage: thioester (if Cysteine) or ester (if Serine)
    • Enzyme: Palmitoyl transferase
    • Motif: not well defined but there‚Äôs a Cysteine involved

    (See slide #18)
  5. Farnesylation Target, Linkage, Enzyme, & Motif
    • Target: C-terminal only (specifically last 4 residues)
    • Linkage: thioether
    • Enzyme: Farnesyl transferase
    • Motif: H3N+... C a a X - COO- (C=Cys, a=aliphatic residue, X = Met, Ser, or Gln)

    (See slide #19)
  6. Farnesyl & Geranylgeranyl transferase uses __ & the target protein as a substrate
    farnesyl diphosphate & geranylgeranyl diphosphate
  7. Geranylgeranyl Target, Linkage, Enzyme, & Motif
    • Target: C-terminal only (specifically the last 4 residues)
    • Linkage: thioether
    • Enzyme: Geranylgeranyl transferase
    • Motif: H3N+... C a a L - COO- (C=Cys, a=aliphatic residue, L=Leu)

    (See slide #19)
  8. Farnesylation & Geranlgeranylation processing
    The 3 residues AFTER Cysteine are cut out leaving Cys as the last residue. The carboxylate end is methylated.
Author
sophathida
ID
351029
Card Set
Lipid anchors
Description
Peripheral membrane protein lipid anchors
Updated