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Myristoylation Target, Linkage, Enzyme, & Motif
- Target: N terminal only
- Linkage: amide
- Enzyme: Myristoyl transferase
- Motif: H2N - M G X X X S...COO-
(See slide #18)
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Myristoyl & Palmistoyl transferase uses __ & the target protein as a substrate
myristoyl-CoA & palmistoyl-CoA
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Myristoylated protein processing
Methionine is clipped off by protease, exposing Glycine. The myristoyl-CoA bond is broken.
Note: No processing is required for Palmitoylation
(See slide #18)
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Palmitoylation Linkage, Enzyme, & Motif
- Target: not specific
- Linkage: thioester (if Cysteine) or ester (if Serine)
- Enzyme: Palmitoyl transferase
- Motif: not well defined but there’s a Cysteine involved
(See slide #18)
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Farnesylation Target, Linkage, Enzyme, & Motif
- Target: C-terminal only (specifically last 4 residues)
- Linkage: thioether
- Enzyme: Farnesyl transferase
- Motif: H3N+... C a a X - COO- (C=Cys, a=aliphatic residue, X = Met, Ser, or Gln)
(See slide #19)
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Farnesyl & Geranylgeranyl transferase uses __ & the target protein as a substrate
farnesyl diphosphate & geranylgeranyl diphosphate
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Geranylgeranyl Target, Linkage, Enzyme, & Motif
- Target: C-terminal only (specifically the last 4 residues)
- Linkage: thioether
- Enzyme: Geranylgeranyl transferase
- Motif: H3N+... C a a L - COO- (C=Cys, a=aliphatic residue, L=Leu)
(See slide #19)
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Farnesylation & Geranlgeranylation processing
The 3 residues AFTER Cysteine are cut out leaving Cys as the last residue. The carboxylate end is methylated.
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