Vagina Normal Micorbiota

  1. What is the gram stain and shape of the most common probiotic, which is normal microbiota of mouth, gut, vagina
    • Gr +
    • Rod
    • Non-endospore former
    • This is lactobacillus
  2. Lactic acid is the major fermentation product of this bacteria, but it can also be involved in the progression of ______, and sometimes also affecting the heart causing _____
    • Dental caries (progression, NOT initiation)
    • Endocarditis
    • This is lactobacillus
  3. When I say pleomorphic, no cell wall, you think:
  4. T/F: most common form of mycoplasma is mycoplasma genitalium
    False; it’s the pneumonia one: mycoplasma pneumoniae
  5. Urogenital mycoplasma organisms:
    • Mycoplasma genitalium
    • Mycoplasma hominis
    • Ureaplasma urealyticum/ ureaplasma parvum
  6. T/F: mycoplasma lack cell membrane
    False; they lack cell WALL, making them pleomorphic. They have cell membrane, and it contains sterols
  7. Mycoplasma cell membranes contains ____, making it hard for them to survive outside of us
  8. Mycoplasma lack _____, making them ______ to penicillin and cephalosporin
    • Cell wall
    • Resistant
  9. T/F: Ureaplasma is urease-positive, but mycoplasma is not
  10. Beta hemolytic, anaerobe, gram variable rod with thin wall:
    Gardnerella vaginalis
  11. Gardnerella exists planktonically as _____
    Biofilms in vagina
  12. Bacterial vaginosis discharge consistency is _____, and the amine odor whiff test _____
    • Thin white, gray discharge
    • Fishy smell
  13. What are clue cells?
    Vaginal epithelial cells that are heavily coated with Gardnerella vaginalis
  14. Beta hemolytic, gram positive cocci, bacitracin resistant:
    Streptococcus agalactiae
  15. What capsule does GBS (S. agalactiae) have?
    Sialic acid capsule
  16. S. agalactiae unique things:
    • CAMP +
    • Hippurate hydrolysis +
    • Bacitracin resistant
  17. Hippurate hydrolysis test for S agalactiae would have the test tube turn:
  18. Virulence factor of S agalactiae:
    • Capsule (sialic acid)
    • C5a peptidase
    • B hemolysin
  19. C5a peptidase is a virulence factor for which bug?
    S agalactiae
  20. Which anatomic GU sites do microbes permanently colonize?
    • Anterior urethra
    • Vagina
  21. Normal microbiota of female anterior urethra:
    • Lactobacilli
    • Streptococci
    • Coagulase negative staphylococci
    • Candida
  22. T/F: newborns are colonized with lactobacilli at birth, and this is normal microbiota of the healthy vagina
Card Set
Vagina Normal Micorbiota
Endo Exam 2