Exam 1

  1. An antibody binding to an antigen is involved with which of the following techniques?
    • Western Blot
    • Immunofluorescence/fluorescent microscopy
  2. This technique separates molecules by size
  3. This technique can analyze the expression of many genes at the same time
    DNA microarrays
  4. This picture shows what technique?
    Western Blot
  5. What is the purpose of Coomassie blue?
    It stains proteins on a gel
  6. What is the purpose of the tubulin in some of the Western Blots in the journal articles we looked at?
    It controls for loading efficiency
  7. Proteins are measured in _____ in protein electrophoresis and Western Blots
    kDa (or Da)
  8. _____ is used in fluorescent microscopy or immunofluorescence. It gives a fluorescence color that can then be used to measure the distribution of proteins in a cell.
  9. Protein electrophoresis is run _____
  10. In either protein or DNA electrophoresis, the large size molecules would be on the _____ of the gel
  11. A dehydration reaction is involved in forming a peptide bond between two amino acids. This statement is
  12. Which of the following amino acids has a side chain that contains an OH group?
  13. Which of the following are features that a model organism should have?
    • Short life span
    • Similar biochemical pathways to humans
  14. Which of the following are true about a hematopoietic stem cell?
    • It is self-renewing
    • It can form all of the blood cells in the body
  15. Which cell type makes antibodies?
    B cells
  16. Which cell type carries oxygen?
    Red blood cells
  17. Which cell type is a macrophage in the brain?
    Microglial cells
  18. Which of the following IS NOT a feature that defines life?
    Ability to use oxygen
  19. _____ is the process of breaking down substances
  20. Which of the following are (is) true about the functional group shown below? O=C-O-H
    • It is involved in a peptide bond
    • It is a carboxylic acid group
  21. Which of the following are (is) true about the functional group shown below? O-P=O
    It is found in a cell membrane
  22. The left end of a protein is labelled _____ and the right end is labelled _____
    • N
    • C
  23. Hydrogen bonds are found
    Between two water molecules
  24. Which of the following assist in the assembly/folding of proteins?
  25. Which of the following bonds are important in the folding of the tertiary structure of a protein?
    • Disulfide
    • Hydrogen bond
  26. _____ bonds involving the _____ are important in stabilizing the secondary structure of many proteins
    • Hydrogen
    • CO and NH of the backbone of the amino acids
  27. The quaternary level of protein structure
    Is when different subunits of a protein bind together
  28. Western Blots, like the ones discussed in class and performed in the lab, use _____ antibody (ies)?
  29. Which of the following are found in BOTH prokaryotic and eukaryotic cells?
    Cell membrane
  30. What is the subunit that makes up proteins?
    Amino acids
  31. A glycosidic bond is found in _____ and it is formed by _____
    • Sugars
    • Joining together two hydroxyl groups in a dehydration reaction
  32. Which of the following amino acids form disulfide bonds?
  33. Ubiquitin targets proteins to the _____ and is involved in protein _____
    • Proteasome
    • Degradation
  34. What is the function of protein kinases?
    To add phosphate groups onto a protein
  35. An amino acid is made up of:
    • Alpha carbon linked to hydrogen
    • Amino group
    • Carboxylic acid group
    • Side chain
  36. In an amino acid, the central carbon
    Forms 4 single bonds
  37. DNA is found in the nucleus of
    Eukaryotic cells only
  38. The _____ has ribosomes attached
    Rough ER (endoplasmic reticulum)
  39. _____ contain catalase and break down hydrogen peroxide
  40. Hydrophobic molecules are
    Found on the inside of a membrane and are non-polar
  41. Peptide bonds are formed between the side chains of amino acids. This statement is
  42. Which of the following is true about ribosomes?
    None of these statements are true
  43. What was the specific purpose for the Western Blot that we performed in class?
    To detect the myosin light chain 1 protein in different fish samples
  44. What was the correct order of steps in the Western Blot that we performed in class?
    • Electrophoresis
    • Transfer to a membrane
    • Add primary antibody
    • Add secondary antibody¬†
    • Add substrate
  45. In the Western Blot that we performed in class, during the electrophoresis step, what buffer did we use?
    TGS- tris glycine SDS
  46. In Western Blot that we performed in class, what was the gel made of?
    Acrylamide or polyacrylamide
  47. Which of the following are model organisms discussed in chapter 1 in your textbook?
    • Yeast
    • E. coli (bacteria)
    • Drosophila (fruit flies)
  48. Which of the following cells has the greatest potential to develop into many other different cells in the body?
    The bone marrow hematopoietic stem cell
  49. Which of the following is a function of proteins?
    Both transport and cell signaling
  50. The alpha-helix is involved with the _____ level of protein structure
Card Set
Exam 1
Chapters 1-4