251 Lab 11

  1. _____-_____ reactions are highly specific reactions in which the antigen binding site of an antibody binds to one antigenic determinant= ?
    • Antigen-antibody
    • epitope
  2. Antigen-antibody reactions include: 7
    • 1. precipitation¬†
    • 2. agglutination
    • 3. neutralization of viruses and toxins
    • 4. immobilization of bacteria
    • 5. complement fixation
    • 6. antibody-dependent cytotoxicity
    • 7. opsonization.
  3. ______ is the discipline that studies antigen-antibody reactions using serum and in vitro diagnostic tools.
    Serology
  4. The use of gel immunodiffusion to study ______, a type of antibody-antigen reaction that occurs between _____ _____ and their respective _____ _____.
    • precipitation
    • soluble antigens
    • specific antibody
  5. (antibody in the serum)
    Antiserum
  6. both solutions diffuse out of the well
    double diffusion
  7. If the serum has a specific antibody that recognizes the soluble antigen, then a ____ ____ ____ will form.
    white precipitin line
  8. The _____ _____ _____ are from HIV virus,Borrelia burgdorferi (the bacterial agent of Lyme disease), and H1N1 influenza virus.
    simulated soluble antigens
  9. When antigenic determinants (which are part of the microbial cell) combine with specific antibodies, visible clumps (aggregates) are produced. This process is called _______
    agglutination
  10. The clumps represent ____ ____ of antigens with various antibodies.
    cross linking
  11. To enhance the observation of clumps in an agglutination reaction,_____ ____ are often used. These beads have specific antibodies _____ to the latex particles.
    • latex beads
    • adsorbed
  12. It is also always necessary to use a _____ and _____ control to verify that the results obtained are not false positive or false negative.
    • positive
    • negative
  13. ____ _______ can be used for a rapid, specific,and definitive identification of this pathogen.
    Slide agglutination
  14. Image Upload 1
    Image Upload 2
  15. Image Upload 3
    • Positive control
    • Negative Control
    • Unknown Sample
  16. Image Upload 4
    • a. Latex bead w/specific anibodies adsorbed
    • b. specific antibody stuck on bead
    • c. Bacterium w/antigenic determinants
Author
ccab1979
ID
346741
Card Set
251 Lab 11
Description
251 Lab 11
Updated