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Eosin Methylene Blue Agar EMB RESULTS
- lactose ferment=metallic green=e.coli
- non-lactose=clear= salmonella
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MacConkey Agar MCA RESULTS
- lactose ferment=pink=e.coli
- non=no color=proteus vulgaris
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Hektoen Enteric Agar HEA RESULTS
- lactose ferment=orange=e.coli
- non=green=salmonellaHydrogen sulfide production=black=salmonella
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selective
These media typically select for the growth of gram (-) bacteria, inhibit gram+
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differential
differentiate lactose fermenters (coliforms) from those gram (-) that do not ferment lactose (non-coliforms).
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Ready cult ingredients
uses indole to show the presence of coliforms in water, shows a blue-green presence in water
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S.aureus does 4 things ___ on Blood agar, also is ___ that allows it to grow on ___ plates, it ferments ___, and produces the _____ enzyme which causes coagulation of plasma by converting _____ to a ____clot.
- hemolytic on blood agar
- halotolerant allowing it to grow on salt plates
- and it ferments mannitol
- coagulase
- fibrinogen
- fibrin
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coagulase +=?
coagulate produce bubbles in Hydrogen Peroxide
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_____are diseases/disorders that occur in a particular community at higher than expected rates.
Epidemics
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(people who study infectious diseases)
epidemiologists
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food infections, may be of a ___ ___ (also called a___ ___ )
- point source
- common source
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In a ____ ___ outbreak, all individuals in the outbreak acquire the infectious disease at one location.Example=
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_____ epidemics are infections that spread from one person to another because the disease is ____ (spread from person to person, a good example of this is____).
- Propagated
- communicable
- influenza
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A _____, such as, H1N1 Swine influenza, tuberculosis, or HIV/AIDS, occurs when an epidemic spreads to more than one continent.
pandemic
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The infectious agent is a bacterium,____ ____, spread through candy and handshaking.
Serratia marcescens
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Your job will be to determine the ____ ___, the individual that was originally infected and spread the infection throughout the class.
index case
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The skin and hands have normal microbiota, both ____ flora, which are temporarily present, and ____ flora, which are entrenched in layers of the skin and always present.
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infectious disease acquired while in the health care setting is termed a ____ ____.
nosocomial infection
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normal flora = Microbes that inhabit the skin must be able to survive slightly ____ ____ conditions and slightly conditions (as low as pH __).
- hypertonic (NaCl)
- acidic
- 4.0, but more generally 5 – 6
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____ ____, a common skin inhabitant, and ___ ____, much less common on the skin but, present in the nares of up to 30% of individuals.
- Staphylococcus epidermididis
- Staphylococcus epidermididis
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transient microbiota can be easily acquired and transferred in a health care setting. These may include methicillin resistant ____ ____ __ which commonly lives in many hospitals, antibiotic resistant gram negative rods, vancomycin resistant ___ ____ and ____ ___ a common nosocomial pathogen which may be spread through spores.
- Staphylococcus aureus(MRSA)
- Enterococcus (VRE)
- Clostridium difficile(C. diff)
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Surgeons scrub hands prior to surgery. They use ____ soap
germicidal
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You will determine the number of bacteria as ____ ___ ___ / ml ineach original hand scrubbing basin. ___/ml is a common unit of measure for determining the number of microbes in samples.
colony forming units (CFU)
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A plate with less than __ colonies is not representative of the true number of colonies and a plate greater than ___ is not accurately counted.
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if your average count is 0.2 ml plates, then multiply the count times?
5
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if your average count is 0.1 ml plates, then multiply the count times?
10
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if your average count is 0.4 ml plates, then multiply the count times?
2.5
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Count both plates of the particular dilution that you choose. ____the two numbers to arrive at the count.
Average
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If the colony count is obviously greater than 300, then record ___ ___ ___ ___ in the table as ___.
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