251 Lab 10

  1. Eosin Methylene Blue Agar EMB RESULTS
    • lactose ferment=metallic green=e.coli
    • non-lactose=clear= salmonella
  2. MacConkey Agar MCA RESULTS
    • lactose ferment=pink=e.coli
    • non=no color=proteus vulgaris
  3. Hektoen Enteric Agar HEA RESULTS
    • lactose ferment=orange=e.coli
    • non=green=salmonella
    • Hydrogen sulfide production=black=salmonella
  4. selective
    These media typically select for the growth of gram (-) bacteria, inhibit gram+
  5. differential
    differentiate lactose fermenters (coliforms) from those gram (-) that do not ferment lactose (non-coliforms).
  6. Ready cult ingredients
    uses indole to show the presence of coliforms in water, shows a blue-green presence in water
  7. S.aureus does 4 things ___ on Blood agar, also is ___ that allows it to grow on ___ plates, it ferments ___, and produces the _____ enzyme which causes coagulation of plasma by converting _____ to a ____clot.
    • hemolytic on blood agar
    • halotolerant allowing it to grow on salt plates
    • and it ferments mannitol
    • coagulase
    • fibrinogen
    • fibrin
  8. coagulase +=?
    coagulate produce bubbles in Hydrogen Peroxide
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  10. _____are diseases/disorders that occur in a particular community at higher than expected rates.
    Epidemics
  11. (people who study infectious diseases)
    epidemiologists
  12. food infections, may be of a ___ ___ (also called a___ ___ )
    • point source
    • common source
  13. In a ____ ___ outbreak, all individuals in the outbreak acquire the infectious disease at one location.Example=
    • point source
    • salmonella
  14. _____ epidemics are infections that spread from one person to another because the disease is ____ (spread from person to person, a good example of this is____).
    • Propagated
    • communicable
    • influenza
  15. A _____, such as, H1N1 Swine influenza, tuberculosis, or HIV/AIDS, occurs when an epidemic spreads to more than one continent.
    pandemic
  16. The infectious agent is a bacterium,____ ____, spread through candy and handshaking.
    Serratia marcescens
  17. Your job will be to determine the ____ ___, the individual that was originally infected and spread the infection throughout the class.
    index case
  18. The skin and hands have normal microbiota, both ____ flora, which are temporarily present, and ____ flora, which are entrenched in layers of the skin and always present.
    • transient
    • resident
  19. infectious disease acquired while in the health care setting is termed a ____ ____.
    nosocomial infection
  20. normal flora = Microbes that inhabit the skin must be able to survive slightly ____ ____ conditions and slightly  conditions (as low as pH __).
    • hypertonic (NaCl)
    • acidic
    • 4.0, but more generally 5 – 6
  21. ____ ____, a common skin inhabitant, and ___ ____, much less common on the skin but, present in the nares of up to 30% of individuals.
    • Staphylococcus epidermididis
    • Staphylococcus epidermididis
  22. transient microbiota can be easily acquired and transferred in a health care setting. These may include methicillin resistant ____ ____ __ which commonly lives in many hospitals, antibiotic resistant gram negative rods, vancomycin resistant ___ ____ and ____ ___ a common nosocomial pathogen which may be spread through spores.
    • Staphylococcus aureus(MRSA)
    • Enterococcus (VRE)
    • Clostridium difficile(C. diff)
  23. Surgeons scrub hands prior to surgery. They use ____ soap
    germicidal
  24. You will determine the number of bacteria as ____ ___ ___ / ml ineach original hand scrubbing basin. ___/ml is a common unit of measure for determining the number of microbes in samples.
    colony forming units (CFU)
  25. A plate with less than __ colonies is not representative of the true number of colonies and a plate greater than ___ is not accurately counted.
    • 30
    • 300
  26. if your average count is 0.2 ml plates, then multiply the count times?
    5
  27. if your average count is 0.1 ml plates, then multiply the count times?
    10
  28. if your average count is 0.4 ml plates, then multiply the count times?
    2.5
  29. Count both plates of the particular dilution that you choose. ____the two numbers to arrive at the count.
    Average
  30. If the colony count is obviously greater than 300, then record ___ ___ ___ ___ in the table as ___.
    • too numerous to count
    • TNC
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Author
ccab1979
ID
346672
Card Set
251 Lab 10
Description
251 Lab 10
Updated