1. What if APP is not up at all?
    • 1) we know APP mRNA is up in the transcriptome in response to motor learning. 
    • 2) if we don't see APP protein up in our experiment, we likely need to add 1 more timepoint (17days) because the time from transcription to translation can take up to 3 days in vivo.
  2. What if APP is not alternatively spliced?
    It is not alternatively spliced in response to learning.
  3. What is GAPDH?
    It is a house keeping gene. House keeping genes are used as a control. For example, if you show APP protein goes up in your western, how do we know you didn't cheat? you use a house keeping gene to show that you loaded equal amount of proteins in each well during a western blot, because house keeping genes usually don't go up or down.
  4. Why do you want to do the BS/MS?
    I want to partake in the BS/MS program because I recently took the MCAT, and I will be applying to medical school this cycle. When I go into medicine one day, I plan to also do some form of research. Hence, by having a master’s degree, it will give me leverage among my class.
  5. Stuff we talked about in your office:
    • Upon training mice, we see an increase in boutons and dendritic complexity.
    • When looking at the transcriptome throughout training, from the interval 1-7 days, we see everything as being fairly neutral. However, at around day 14, we see an upregulation. For this reason, we are interested to know if APP plays a role in motor learning in healthy subjects. APP does play a role in Alzheimer’s, a disease where memory loss is an associated pathology.
    • Experiments:
    • 1) Forelimb reach to grasp task – focusing on days 1, 4, 7 & 14
    • 2) Extract the APP protein and subject it to western blot analysis
    • 3) Histology
    • ) Perfusion – fixative to replace blood and fix tissue
    • ) Tissue processing by cutting the brain
    • ) Immunolabeling - antibodies against APP, then we will use Neun = Neuron Marker and a retrograde tracer
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