Enteracteriaceae I and yersinia

  1. What are the (3) lipopolysaccharide (LPS) antigens on the cell wall of enterobacteriaceae
    • O polysachharide/ O antigen
    • core polysaccharide
    • lipid a
  2. what is significance of O Polysaccharide/ O antigen. What bacteria is it found in
    differs between strains of same species of enterobacteriaceae
  3. what is the significance of core polysaccharide
    distinct in enterobacteriaceae
  4. what is the significance of lipid A
    endotoxin activity. strong immune response
  5. what are the serologic and epidemiologic classification of enterobacteriaceae
    • somatic O polysaccharide
    • K antigen
    • H protein
  6. what is somatic O polysaccharide
    present in every genus and species of enterobacteriaceae
  7. what is K antigen
    • found in capsules of enterobacteriaceae. 
    • not commonly used for strain typing
    • may interfere with detection of O antigens
  8. what is H protein
    heat labile proteins in bacterial flagella of certain enterobacteriaceae
  9. what are the pathogenesis of enterobacteriaceae
    • endotoxin (lipid A of LPS)
    • capsule
    • antigenic phase variation
    • Type III secretion system (T3SS)
    • antimicrobial resistance
  10. what is type III secretion system (T3SS)
    protein complex that acts as a syringe to secrete virulence factors to host cells.
  11. what immune response does enterobacteriaceae endotoxin cause?
    • fever
    • vasodilation and capillary leakage
    • inflammatory response
    • septic shock
    • DIC
    • death
  12. yersinia pestis causes what type of pathogen
    plague, a systemic disease
  13. yersinia enterocolitca causes what type of pathogen
    enterocolitis, a gastrointestinal disease
  14. yersinia pseudotuberculosis causes what type of disease
    • pseudotuberculosis in animals
    • enterocolitis in humans
    • rare scarlatinoid fever
  15. what type of protein complex does yersinia have that help them escape phagocytic killing
    Type III secretion system (T3SS)
  16. what is the name of the virulence plasmid that encodes for yersinia T3SS
    Yop plasmid (yersinia outer proteins)
  17. what are the types of proteins secreted by yersinia t3SS
    • Yop H
    • Yop E
    • YopJ/ Yop P
  18. what does Yop H do
    disrupts host proteins required for phagocytosis
  19. what does Yop E do
    activates cell death pathway by disrupting actin filaments
  20. what does Yop J/ Yop P do
    initiate apoptosis (cell death) in MQ and suppress cytokine production
  21. what are the virulence factors of Y. pestis (2)
    • fraction 1(F1): anti-phagocytic protein capsule
    • plasminogen activator protease (Pla): prevents opsonization, phagocyte migration, and degrades fibrin clots to facilitate bacterial spreading
  22. what are the types of plague caused by Y. pestis (4)
    • urban plague: spread by rat fleas
    • sylvatic plague: spread by squirrels, groundhogs (wild animals)
    • bubonic plague: infection of lymph node in humans, no direct human to human spread of organism
    • pneumonic plague: person to person through aerosols
  23. Y. pestis is aquired from
  24. Y. enterocolitica is acquired from and causes
    • contaminated food exposed to livestock, rodents
    • enterocolitis: watery diarrhea
  25. y. pseudotuberculosis is acquired by
    • contaminated foods from rodents, wild animals, game birds
    • rarely causes disease in humans but can cause enterocolitis symptoms similar to y. enterocolitica
  26. what agar selects Y.enterocolitica
    CIN agar (cefsuldodin irgansan novobiocin)
  27. what kind of colonies do yersinia typically produce on CIN agar?
    bull's eye appearance with red center due to fermentation of mannitol
  28. what are the enteric bacteria tests for yersinia
    • KLUMCI
    • API strip
    • serotyping
  29. what are the specimens where yersinia can be collected from
    • blood
    • bubo pus
    • sputum
Card Set
Enteracteriaceae I and yersinia
characteristics of enterobacteriaceae and yersinia