name bases and nucleoside nomenclature
Which of the following is not true regarding ATP?
A.It has a 2’ OH group
B.It acts as energy currency in the cell due to the high energy pentose/adenine glycosidic bond
C.It is generated by the cell primarily during oxidative phosphorylation
D.It is not incorporated into a nucleotide chain by DNA polymeras
E.All of the above are true
what keeps a DNA helix together?
Which of the following best describes the directionality of a DNA strand?
A. Only one end of a strand has a free phosphate and one a free OH
How do you accomplish DNA denaturation?
DNA undergoes a hyperchromic shift during _____?
Tm is what?
temperature at which the rise in A260 id half complete
How would the following changes affect Tm of a given DNA molecule?1. Replace ½ of the A-T nucleotides for G-C
Forces that stabilize the helix:
1.composition:stacking and H-bonds
2.electrostatics:effect of salt composition
How would the following changes affect Tm of a given DNA molecule?2. Increase pH from neutral to basic
How would the following changes affect Tm of a given DNA molecule?3. Double the salt concentration in the solution
what is nucleic acid hybridization useful for?
useful to locate gene sequences
why is DNA our genetic information, not RNA?
more stable genetic form
RNA has -OH (the problem)
-strong nucleophile, will attack the phosphate
-strong bases catalyzes this reaction as well as Pb+, Hg+