Acid fast stain is a differential stain that detects the presence or absence of ______ _____ in the bacterial cell wall.
mycolic acids
What genera and species is mycolic acid found in?
Myobacterium
Nocardia
allowing survival of Mycobacterium in phagocytes, and contributing to resistance to disinfectants and antimicrobial therapy and may make a mycobacterial infection more difficult to treat, is called?
Virulence Factor
The _______method is a cold acid fast stain method using a phenolic compound,carbol fuchsin as the primary dye which is a lipid soluble, concentrated stain.
Kinyoun
Primary Dye in Acid Fast Stain? Time?
Carbol Fuchsin
5 mins
Mordant in acid Fast stain?
Phenol
Decolorizer in Acid Fast Stain? Time?
Acid Fast Alcohol
10-15 seconds
Counter stain in acid fast stain? Time?
Methylene Blue
30 seconds
The acid fast stain is used as a _________test for the presence of acid fast bacteria in clinical specimens where infections with Mycobacterium are suspected.
presumptive
causes tuberculosis?
Mycobacterium tuberculosis
What type of infection is TB?
re-emerging
_______ ______ causes leprosy and is also acid fast but is much less common than tuberculosis.
Mycobacterium leprae
Read ______rods as acid fast positive. Read _____cocci as acid fast negative.