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Not known to cause disease in healthy humans
BSL1
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Associated with human disease in healthy but easily contained. Transmission through percutaneous, ingestion, mucous membrane
BSL2
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Disease may have serious or lethal consequences
BSL3
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When do you wash hands? and where?
- Before and after lab
- in lab
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Inform the instructor of microbial spills immediately, then?
- Cover culture spills with paper towels.
- Soak with disinfectant immediately,
- let stand for 10 minutes,
- re-clean with new paper towel and,
- dispose of all towels in autoclave bag.
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How are lab coats stored?
In a plastic bag with name, then in the class bin to be autoclaved for next week
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When should goggles be worn?
When working with liquid cultures
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What is the procedure for a fire?
- Inform professor
- Evacuate area
- Pull fire alarm
- Inform CSN security
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3 domains all living organisms are grouped into
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Microorganisms are _________, i.e., they can be found in every habitat on earth
ubiquitous
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Solid media contains ______(derived from seaweed) in addition to the nutrients.
agar
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________ ________ media allow the growth of many microorganisms that do not need special growth factors
General purpose
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_________ media contain special _____ _____ required by some microorganisms to grow.
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_________ media is used to inhibit the growth of some groups of microorganisms and select for the growth of other microorganisms.
Selective
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Example of Selective Media
Sabouraud’s dextrose agar
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_________ media show differences between microbial colonies based on their cultural characteristics (what they look like when growing in colonies).
Differential
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What 2 medias is Blood agar an example of?
- Differential Medium
- Growth Factor Media
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Partial hemolysis of red blood cells, green brown coloring
Alpha Hemolysis (α)
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Complete lysis of red blood cells, eat agar
Beta Hemolysis
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colonies that are not hemolytic called? No reaction in surrounding medium
Gamma Hemolysis
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distinct and separated from other similar masses, it is called a _________(clones from one bacterium)
colony
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_________ _________, which are present in healthy individuals
normal microbiota
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used as a nutrient source to allow microbes to grow
Culture Media
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Discuss solid culture media. What makes it solid? What is the advantage for microbialgrowth.
- Solid is solid at room temp
- provides a flat, solid surface for inoculating and growing microorganisms
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property whereby an image in focus in a lower power will remain in focus when changing to a higher power objective
parfocal
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ability to see 2 points as separate and distinct under the microscope?
resolution
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used with objective (100x) reduces this bending and distortion
Immersion oil
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Condenser Lens Adjustment Knob
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