-
Not known to cause disease in healthy humans
BSL1
-
Associated with human disease in healthy but easily contained. Transmission through percutaneous, ingestion, mucous membrane
BSL2
-
Disease may have serious or lethal consequences
BSL3
-
When do you wash hands? and where?
- Before and after lab
- in lab
-
Inform the instructor of microbial spills immediately, then?
- Cover culture spills with paper towels.
- Soak with disinfectant immediately,
- let stand for 10 minutes,
- re-clean with new paper towel and,
- dispose of all towels in autoclave bag.
-
How are lab coats stored?
In a plastic bag with name, then in the class bin to be autoclaved for next week
-
When should goggles be worn?
When working with liquid cultures
-
What is the procedure for a fire?
- Inform professor
- Evacuate area
- Pull fire alarm
- Inform CSN security
-
3 domains all living organisms are grouped into
-
Microorganisms are _________, i.e., they can be found in every habitat on earth
ubiquitous
-
Solid media contains ______(derived from seaweed) in addition to the nutrients.
agar
-
________ ________ media allow the growth of many microorganisms that do not need special growth factors
General purpose
-
_________ media contain special _____ _____ required by some microorganisms to grow.
-
_________ media is used to inhibit the growth of some groups of microorganisms and select for the growth of other microorganisms.
Selective
-
Example of Selective Media
Sabouraud’s dextrose agar
-
_________ media show differences between microbial colonies based on their cultural characteristics (what they look like when growing in colonies).
Differential
-
What 2 medias is Blood agar an example of?
- Differential Medium
- Growth Factor Media
-
-
Partial hemolysis of red blood cells, green brown coloring
Alpha Hemolysis (α)
-
-
Complete lysis of red blood cells, eat agar
Beta Hemolysis
-
colonies that are not hemolytic called? No reaction in surrounding medium
Gamma Hemolysis
-
-
distinct and separated from other similar masses, it is called a _________(clones from one bacterium)
colony
-
-
_________ _________, which are present in healthy individuals
normal microbiota
-
used as a nutrient source to allow microbes to grow
Culture Media
-
Discuss solid culture media. What makes it solid? What is the advantage for microbialgrowth.
- Solid is solid at room temp
- provides a flat, solid surface for inoculating and growing microorganisms
-
property whereby an image in focus in a lower power will remain in focus when changing to a higher power objective
parfocal
-
ability to see 2 points as separate and distinct under the microscope?
resolution
-
used with objective (100x) reduces this bending and distortion
Immersion oil
-
-
-
-
-
-
Condenser Lens Adjustment Knob
|
|