Microbiology

  1. Intracellular Bugs
    • Obligate: Rickettsia, CHlymidia, COxiella
    • @Stay inside cells when it is Really CHilly and COld
    • Facultative: Salmonella, Neisseria, Bordetella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia
    • @Some Nasty Bugs May Live Facultative IntracellularLY

  2. Encapsulated Bacteria
    • Strep pneumoniae, Klebsiella pneumoniae, Hemophilus influenzae type b, Pseudomonas aeruginosa,Neisseria meningitidis, Cryptococcus neoformans (fungi)
    • @ Some Killers Have Pretty Nice Capsules

    Capsular polysaccharide plus protein conjugate serves as an antigen in vaccines
  3. Giemsa stain
    • Rickettsia, Chlamydia, Trypanosomes, Plasmodium, Borrelia
    • @Ricky got CHLAMYDIA as he Tryed to Please Bored Giemsa

  4. PAS
    • Stains glycogen, mucopolysaccharides
    • Used to diagnose Whipple disease (Tropheryma whipplei)
    • @PASs the Sugar

  5. Obligate anaerobes
    • Actinomyces, Bacteroides, Clostridium, Fusobacterium
    • @ABC
    • @Anaerobes Cant Breathe Fresh Air
    • Normal flora of GIT, pathogen elsewhere
    • AminOglycosides are ineffective against anaerobes as these require Oto enter into bacterial cell.
  6. Obligate aerobes
    • Nocardia, Pseudomonas, MycoBacterium
    • @Nagging Pests Must Breathe
    • Do not ferment sugar
    • Consist of superoxide dismutase to fight oxidative stress
  7. Thayer Martin agar
    • Chocolate agar with antibiotics
    • Selective growth of Neisseria
    • Vancomycin inhibits gram +
    • Trimethoprim and Colistin inhibit gram - except Neisseria
    • Nystatin inhibits fungi
    • @Very Typically Cultures Neisseria
  8. Culture media
    • Hemophilus influenzae: Chocolate agar with factor V (NAD+) and factor X (hematin)
    • Bordetella pertussis: Bordet-Gengou, Regan-Lowe
    • C. diptheriae: Tellurite agar, Loffler medium
    • M. tuberculosis: Lowenstein-Jensen agar
    • Mycoplasma Pneumoniae: Eaton agar
    • Lactose fermenting enterics: Mac-Conkey agar
    • E. coli: Eosin Methylene Blue (EMB) agar
    • Legionella: Charcoal Yeast Extract (CYE) agar bufferred with cysteine and iron
    • Fungi: Sadourad's Dextrose agar @Sab's a fun guy!
  9. Urease Positive Organisms
    • Proteus, Cryptococcus, H. pylori, Ureaplasma, Nocardia, Klebsiella, S. epidermidis, S. saprophyticus
    • @ Pee CHUNKSS
    • Predisposes to struvite (ammonium magnesium phosphate) stones, particularly Proteus
    • Urease hydrolyzes urea to release ammonia and CO2 ↑pH



  10. Catalase Positive Organisms
    • Catalase degrades H2O2 into H2O and bubbles of O2
    • People with CGD (NADPH oxidase deficiency) have recurrent infections with catalase positive organisms
    • Nocardia, Pseudomonas, Listeria, Aspergillus, Candida, E.coli, Staphylococci, Serratia, Burkholderia cepacia, H. pylori
    • @ Cats Need PLACESS to Belch their Hairballs
  11. Pigment producing bacteria
    • Actinomyces israeliiyellow sulfur granules, which are composed of filaments of bacteria @ Israel has yellow sand
    • Staph aureus: yellow pigment @ Aureus (latin)= gold 
    • Pseudomonas aeruginosa: blue-green pigment (pyocyanin- blue; pyoverdin- green)
  12. Bacterial toxins encoded by lysogenic phage
    • @COBEDS
    • Cholera toxin: Vibrio cholera
    • O antigen: Salmonella
    • Botulinum toxin: Clostridium botulinum
    • Diptheria toxin: Corynebacterium diptheriae
    • Shiga toxin: Shigella
    • Exotoxins A-C (Erythrogenic/Pyrogenic): Streptococcus pyogenes
Author
Binita
ID
342599
Card Set
Microbiology
Description
Concise microbiology
Updated