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What is Transpiration?
Loss of water vapour from leaves and other aerial parts of the plant.
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What are the parts of a leaf?
- Blade: The leaf part
- Petriole: The connection of the blade to the stem
- Stem: The stem
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What are the parts of a leaf but more detailed?
- Cuticle
- Epidermis
- Xylem
- Phloem
- Palisade Mesophyll
- Spongy Mesophyll
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What is the cuticle?
Protects the plant against water loss and insect invasion.
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What is the epidermis?
Protects the plant
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What is the xylem?
- Is the specialized water conducting tissue of terrestrial plants.
- Composed of many cell types (tracheids and vessel elements)
- Carries water to the leaves.
- Supports the plant.
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What are vessel elements?
- Found in xylem.
- Evolved after tracheids as the water transportation structure that makes up xylem.
- Water moves laterally along a long stretch where the two vessels, the ending and the continuing are run side by side.
- Pores allow rapid water transport.
- Found only in angiosperms.
- Secondary walls are lignified, primary walls include pits and pores that allow water to move laterally.
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What is Lignin?
Organic complex that strengthens the cell walls of vascular plants, waterproofs and protects against pathogens.
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What are tracheids?
- Found in xylem.
- Evolved first.
- Found in all plants.
- Are single celled and shorter than vessel elements.
- They have pits and modified pits along their walls to allow water to move between cells.
- Is a hollow cell with no cytoplasm or nucleus.
- Dead and devoid of protoplast
- !!!! its head is like a condom so it’s less efficient
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How are mineral ions transported in plants?
- Minerals must be transported along with water. There are three major processes that allow mineral ions to pass from the soil to the root.
- Diffusion of mineral ions and mass flow of water carries minerals (passive flow through mass or bulk flow)
- Action of fungal hyphae (fungi gain carbon, plant gains mineral ions and increased surface area.) MYCORRHIZA
- Active transport of K, NO3 or PO3 using the ATP and O2 produced by root hairs through protein pumps
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Explain roots?
- Provides mineral ions and water uptake for the plant
- Successful due to root hairs and extensive branching.
- Zone of maturation, zone of elongation (G), zone of cell division (apical meristem) (M)
- Root hairs: higher solute concentration for osmosis and increased surface area
- Root cap: protects apical meristem
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Root hairs?
higher solute concentration for osmosis and increased surface area
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What is the phloem?
Carries the products of photosynthesis to the other areas of the plant.
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What is the Palisade mesophyll?
Densely packed cylindrical cells carry a large number of chloroplasts. (on the top of the plant)
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What is Spongy Mesophyll?
Loosely packed cells with few chloroplasts. Air spaces provide gas exchange surfaces. There are stomata and guard cells right underneath
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Explain stomata and guard cells.
- Stomata can only close on a short term basis. The opening and closing is controlled by turgor pressure of guard cells with uneven cell wall thickness.
- Blue light triggers ATP powered proton pumps in the guard cells and active transport of K+ into the cell occurs. This causes inward water movement through osmosis and increases turgor. Abscisic acid makes K+ and water diffuse out.
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What is abscisic acid?
Produced in the roots during droughts. Makes K+ and water diffuse out of the guard cells and closes stomata.
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What does transpiration do?
- Cools down the plant and sun soaked leaves and stems
- Allows gas exchange
- Provides minerals to the plant through bulk flow
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Cohesion-Tension-Adhesion Theory of Plant Fluid Movement
- Continuous column of water is maintained,
- Water moves down concentration gradient from leaf to the atmosphere.
- Water lost through transpiration is replaced by water in the vessels (air spaces in the leaf have high water vapour concentration)
- The vessel water column is maintained by cohesion (H bonds between water molecules) and adhesion (H bonds between the water molecules and xylem)
- Tension occurs in the columns of water in the xylem
- Water is pulled from root cortex to xylem cells
- 6. Water is pulled from the soil into the roots
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Stomata and rising CO2 levels?
Lower stomatal density has been observed due to increased CO2 levels in the atmosphere.
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How does light affect transpiration?
Speeds it up by opening stomata.
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How does humidity affect transpiration?
Decreases it, concentration gradient is less.
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How does wind affect transpiration?
Speeds it up, humid air around stomata is carried away
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How does temperature affect transpiration?
Increases it, more water evaporates.
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How does soil water affect transpiration?
Soil water must keep up with transpiration rates. If the water in the soil is insufficient, turgor will be lost and the stomata will close.
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How does CO2 levels affect transpiration?
High Co2 levels cause turgor loss and decreased transpiration. (biological pathways are activated when CO2 falls so stomata opens)
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Xerophytic adaptations?
- Arid climates
- Small thick leaves to reduce transpiration.
- Reduced number of stomata
- Stomata are located in crypts or pits on the leaf surface for higher humidity near stomata
- Thickened waxy surface reduces water loss (trichomes)
- Hair like cells on the leaf surface trap water, increasing humidity
- Become dormant or shed their leaves during dry months
- Succulents store water
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What are trichomes?
Hair like structures that reflect light and secrete chemicals.
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What are halophytic adaptations?
- High salinity
- Succulent to dilute concentrations
- They secrete salt through salt glands
- Can compartmentalize Na+ and Cl- in vacuoles.
- Thick leaves and sunken stomata reduce water loss
- Surface area of leaves is reduced.
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What Are different photosynthesis processes?
- C3 is the most common
- CAM is when stomata are closed during the day and CO2 is incorporated at night.
- C4 is when stomata are open during the day but there is rapid CO2 intake.
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What are the differences between xylem and phloem?
- Xylem transports in one direction, phloem can transport in both directions.
- Xylem is made of dead cells, phloem is made of alive cells.
- In xylem, a continuous tube of dead cells an unbroken column of water, the movement is passive. In phloem, the cells are alive and they enable substances to be loaded by active transport.
- In xylem, the walls are thickened by lignin to withstand negative pressure. In phloem, companion cells carry out cell functions and supply energy for active transport into phloem
- Xylem transports water and minerals passively from roots to leaves. Phloem transports sugars, RNA and hormones to all parts of the plant by mass flow.
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Phloem Structure:
- Sieve tube members: lack nucleus and cytoplasm
- Companion cells: has a nucleus and dense cytoplasm
- These two are connected through plasmodesmata
- Sieve plates: are between different sieve tube members, has pores
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What is the action of phloem called?
Translocation from source to sink
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What is the source in a plant?
Parts of a plant where starch is hydrolyzed or glucose is produced.
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What is the sink in a plant?
Where sugars are either produced or consumed.
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Can a part of a plant be both a source and sink?
Yes, a tube or bulb act as sinks in the summer and sources in early spring.
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What is plant exudate?
- Any fluid that moves out of the normal transport system of plants.
- Resin, gum, saps, latex
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What is phloem sap?
- Solution of water in which organic molecules are dissolved.
- Carries sugars, amino acids, plant hormones, RNA (plant communication)
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Explain translocation:
- At source, sugars are loaded into sieve tube using active transport (using specialized proteins called cotransport, proton pumps) chemiosmotic process. This causes osmosis from surrounding cells
- Uptake of water at source causes positive hydrostatic pressure
- Raised hydrostatic pressure causes the phloem sap to move passively to sink
- Sugars are removed at sink (active transport, cotransport proteins) Sugars are changed to starch at sink which exerts no osmotic pressure
- Xylem recycles relatively pure water, carrying it from the sink back to the source
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What’s so special about phloem anyway?
- Its walls are rigid so hydrostatic pressure is achieved.
- Phloem tissue occurs in all parts of the plant, not just the stem.
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What are companion cells?
- Phloem
- The provide “life support” for the sieve element cells. Plasmodesmata connects them to sieve tube elements.
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What are sieve tube elements?
- Phloem
- No nucleus, ribosomes or vacuoles, reduced cytoplasm (requires companion cells to stay alive)
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What provides support to the phloem?
Fibers of sclerenchyma cells
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What is parenchyma?
It is the packing material between other cell types and and helps transfer materials to the sieve elements and companion cells.
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How can we investigate phloem contents?
- Radioactively tagged compound C
- Autoradiography
- Or we can use aphids
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Cross Section through a stem?
- Cortex: Made mostly of parenchyma cells, unspecialized cells between epidermis and endodermis, has chloroplasts
- May store resin, latex etc.
- Xylem - Cambium (between the two from which they grow) - Phloem
- Pith: Soft, spongy parenchyma cells that transport nutrients.
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Where are phloem and xylem located?
Phloem outside xylem inside
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Most plants are made of three types of tissues:
- Dermal: epidermis
- Ground: parenchyma
- Vascular: xylem + phloem
- All of these are derived meristematic tissue
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What is meristematic tissue?
- Composed of aggregates of small cells that have the same function as stem cells.
- When cells divide, one of the always remains meristematic and one differentiates into a derivative so indeterminate growth is achieved.
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Plant’s life cycle
- Annuals: 1 year
- Biennials: 2 years
- Perennials: many years, usually die of infection or env. Factor
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Types of meristems?
Apical and lateral
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Apical Meristems?
- Primary growth in length
- At the tip of the root or the tip of the shoot
- Results in herbaceous non-woody stems and roots
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What is shoot apex?
Apical meristem and the surrounding development tissue cause primary growth (height) through mitosis and cell division.
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Lateral Meristems?
- Secondary growth
- Allows the growth in thickness
- Develops from the main stem as shoots and branches
- Allows competition and taking advantage of favorable conditions
- Trees and shrubs have two different kinds of lateral meristems:
- Vascular cambium: between xylem and phloem, produces secondary xylem (wood) and phloem
- Cork cambium: produces cork cells for the outer bark
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Meristems with examples?
- Shoot apical meristems
- Root apical meristems
- Vascular cambium (lateral meristem)
- Cork cambium (lateral meristem)
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Which factors affect the development of a plant?
- Environmental factors
- Receptors
- Genetics
- Hormones
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What are receptors?
- Specific cells have proteins in their plasma membrane, cytoplasm or nucleus that allow them to receive environmental stimuli
- Proteins activate upon specific stimuli and allow for different metabolic pathways
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Differences between plant and animal hormones?
- Plant hormones affect various tissues differently.
- Plant hormones require a great deal of interaction within themselves for the appropriate response
- Plant hormones are produced everywhere in the plant,
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What are auxins?
Complex set of hormones that only work with auxin receptors. Found in embryos of seeds, young leaves and shoot apex.
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What are the roles of auxins?
- Phototropism
- Stimulation of cell division (in most meristematic tissue)
- Promotion of elongation of cells in shoot apex.
- Differentiation of xylem and phloem
- Development of lateral roots
- Gene expression (activating, inhibiting genes)
- Influences general pattern of development
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What is tropism?
- Growth in the direction or away from external stimuli (positive or negative)
- What is phototropism?
- In response to light
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What is geotropism?
In response to gravity.
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Explain Phototropism
- Auxins are produced on the tip of the stem (shoot apex) but are translocated to areas with less sunlight. Using Auxin Efflux Carriers. (specialized membrane proteins)
- Higher concentrations of auxins on the B side trigger cell elongation and allow for growth in the direction of the sun.
- The binding of auxins to auxin receptor cells produce specific proteins to ensure a faster growth rate on the B side.
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What is auxin efflux?
The entrance of auxin into the cell. Triggers the production of IAA auxin that stimulate plant growth.
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Explain the Efflux pump action of auxins
- Auxins bind to auxin receptor cells
- Protopump activates (H ion pumps)
- H ions are pumped to the shady side
- The increase in pH results in the breakdown of cellulose fibers in the cell wall
- Cell wall elongates
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How does auxin affect cell growth?
Changes the pattern of gene expression through interacting with the repressor of a particular gene.
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What is micropropagation?
- Cloning of plants
- If you put meristematic tissue in uygun environment and give it auxin, it will grow into a callus. Gibberellins, cytokinin are used to grow the callus into a plant with a stem + roots.
- Agar Gel
- You can bulk up new species p quickly
- Save the plants from virus
- Rare plants could be reproduced instead of harming ecosystems and taking them from nature.
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Differences between monocotyledon and dicotyledon plants?
- Venation:Monocotyledon have parallel venation, dicotyledon have netlike venation pattern in leaves.
- Flower Parts: Three flower parts or multiples of three in monocotyledon, 4 or 5 parts or multiples of four or five in dicotyledon
- Seeds: Monocotyledon only one cotyledon, dicotyledon has two
- Vascular bundles: monocotyledon is arranged all throughout the stem, dicotyledon they’re arranged in a ring
- Root system: monocotyledon is fibrous, dicotyledon has a taproot, main root
- Pollen grain: One opening in monocotyledon, three openings in dicotyledon
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Angiosperm types?
Monocots, manglids (magnolias), eudicots (true dicots)
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Parts of an angiosperm flower?
- Anther: part of the stamen that produces male sex cells
- Filament: holds up stamen
- make up stamen
- Stigma: sticky top of the carpel on which the pollen lands
- Style: supports the stigma
- Ovary: base of the carpel where the female sex cells develop
- Make up carpel
- Petals attract pollinators
- Sepal protects buds
- Receptacle is the booty of the plant
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Different kinds of flowers?
- Complete flowers
- Incomplete flowers (lacks at least 1 part like grass)
- Staminate flowers (no carpels just stamens)
- Carpellate flowers (no stamens just carpel)
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What is pollination?
The process by which pollen (containing male sex cells) is placed on the female stigma
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Kinds of Pollination?
- Cross-pollination (more genetic variety, increased strength, however harder because female stigma might not receive pollen)
- Self Pollination
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What are pollen vectors?
- They carry pollen
- Wind
- Insects
- Water
- Bird, rats
- Mutualistic relationship to the point of co-evolution between flowers and insects.
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What is a pollen tube?
A hollow tube that develops from a pollen grain when deposited on the stigma of a flower.
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Definition of Fertilization?
- Happens when female sex cells are fertilized by the pollen to form a zygote.
- Occurs in the ovule of a flower.
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Explain the process of fertilization?
- Pollen germinates to produce a pollen tube. The pollen tube grows along the style of the carpel.
- Pollen tube acts as conduits of transport for the male gamete cells from the pollen to the ovule. Within the pollen tube is the nucleus that will produce the sperm.
- The pollen tube completes its growth by entering the bottom of the ovary.
- The sperm moves from the tube to combine with the egg of the ovule to form a zygote.
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Fertilization in angiosperm is special?
Yes, it’s actually a double fertilization. After pollination, one of the two sperms produced by the pollen grain combines with the egg. The other combines with two polar nuclei in the ovary to produce a triploid (3n) endosperm.
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Pollination vs Fertilization
- Pollen landing on stigma is pollination
- The male gamete fertilizing the ovule is fertilization
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What is a seed?
- A structure that protects the embryo of the plant
- The seeds will be transported to another location in order to not compete for resources with the parents.
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What happens after fertilization and a seed is formed?
- Dehydration: in order to achieve dormancy
- Dormancy: the seed remains dormant until favorable conditions arise
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Seed Structure?
- Testa: the tough outer coat (outer coat)
- Cotyledons: Seed leaves that function as nutrient storage structures (the meat)
- Hilum: sagdaki cikinti, scar where the seed was attached to ovary
- Micropyle: Scar where the pollen tube entered the ovary
- Embryo root and shoot: become the new plant when germination occurs
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What is germination?
Development of the seed to a functional plant
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What does germination depend on?
- Optimal temperature
- Water (rehydration of enzymes to break down food stores)
- Oxygen: essential for aerobic respiration
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Germination stages and early growth?
- Imbibition: the absorption of water into the seed
- The water triggers the release of growth hormones gibberellin
- Gibberellin triggers the production of amylase
- The breakdown of starch is stimulated by the cells in the aleurone layer
- Starch is hydrolyzed into maltose
- Maltose is converted into glucose and is transported to the embryo for the radicle (embryo root) and plumule (embryo shoot)
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What is embryo root?
Radicle
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What is embryo shoot?
- Plumule
- Divided to two
- Epicotyl: above the attachment site of the cotyledon
- Hypocotyl: below the attachment sites of the cotyledon
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How is flowering controlled?
A change in the expression of genes in the shoot apex
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What is photoperiodism?
- Plant’s response to light involving the relative lengths of day and light
- Continuous darkness
- Plants are able to detect the presence, direction, wavelength, intensity of light
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Different kinds of plants according to light
- Long-day plants
- Short-day plants
- Day-neutral plants
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How is photoperiodism controlled?
- By PHYTOCHROMES
- So, there are two types of phytochromes:
- Inactive (Pr)
- And active (Pfr)
- Red light converts Pr to Pfr, Pfr can absorb far red lights and rapidly turns back to Pr in daylight. However, in darkness, the conversion is really slow. The buildup of Pfr times the dark period. In long day plants, the remaining Pfr after a short night stimulates flowering. In short day plants, Pfr acts as an inhibitor to flowering.
- Pfr activates certain genes in the shoot apex, resulting in changes in gene expression.
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Visible light?
450 (blue) to 600 (red)
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