BIOL 2510 Blood Lab

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    Lymphocyte (B-cell)
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    Lymphocyte (t-cell)
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  8. Leukocytes from most to least common
    • Neutrophils (Never)
    • Lymphocytes (Let)
    • Monocytes (Monkeys)
    • Eosinophils (Eat)
    • Basophils (Bananas)
  9. Agranulocytes-
    lymphocyte & monocyte (lack cytoplasmic granules)
  10. Granulocytes-
    Neutrophil, Basophil, & Eosinophil (contain granules for chemical defense)
  11. Leukocytes- General Function
    Defense and Immunity
  12. Neutrophil normal range
    3000-7000 cells/mm³ blood
  13. Lymphocyte normal range
    1500-3000 cells/mm³ blood
  14. Monocyte normal range
    100-700 cells/mm³ blood
  15. Eosinophil normal range
    100-400 cells/mm³ blood
  16. Basophil normal range
    20-90 cells/mm³ blood
  17. Platelets (thrombocytes) normal range
    150,000-400,000 cells/mm³ blood
  18. Neutrophil function:
    During an acute infection, phagocytosis of bacteria and fungi followed by enzymatic destruction
  19. Lymphocyte T-cell function:
    Attack viruses & tumors
  20. Lymphocyte B-cell function:
    When stimulated by bacteria or toxins, they differentiate into plasma cells. These plasma cells then produce and secrete antigen-specific antibodies
  21. Monocyte function:
    Phagocytosis:Differentiate into macrophages in tissues for phagocytosis (injects and removes debris such as bacteria pathogens from the body)
  22. Eosinophil functions:
    • Attack parasitic worms
    • Play a complex role in allergy and asthma
    • Lessens the severity of allergic reactions
  23. Basophil functions:
    • Releases histamine and other mediators of inflammation
    • Contains Heparin (blood thinner)
    • Increases allergic reactions
  24. Largest Leukocyte:
  25. Not true cells –formed from the breakup of large cells called Megakaryocytes
    Platelets (Thrombocytes)
  26. Platelets (thrombocytes) functions:
    • Seal small openings in blood vessels
    • Instrumental in blood clotting. When bleeding occurs, the platelets swell, clump together, and form a sticky plug that helps stop the bleeding
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    • Cell A: Erythrocyte
    • Cell B: Platelet(Thrombocyte)
    • Cell C: Leukocyte(Monocyte)
    • Cell D: Leukocyte(Neutrophil)
  28. Complete Blood Count (CBC) includes:
    • Total & Differential WBC count
    • Red blood cell (RBC) count
    • Hematocrit (HCT, packed cell volume, PCV)
    • Hemoglobin (Hgb)
    • Red blood cell indices.
    • mean corpuscular volume (MCV),
    • mean corpuscular hemoglobin (MCH),
    • mean corpuscular hemoglobin concentration (MCHC).
    • Platelet (thrombocyte) count
    • Mean platelet volume (MPV)
    • a blood smear test
  29. WBC count normal range:
    4800-10,800 WBC/mm³ blood
  30. RBC count normal range:
    • Males = 5.1-5.8 million cells/mm3
    • Females = 4.3-5.2 million cells/mm3
  31. Hematocrit (HCT) calculation:
    Divide the height of the erythrocytes by the total height of the blood in the tube and multiply by 100.
  32. Hematocrit (HCT) normal range:
    • males- 42-52%
    • females- 37-47%
  33. Hemoglobin (HGB) normal range:
    • Male 13-18 g/dl
    • Female 12-16 g/dl
  34. Mean corpuscular (RBC) volume (MCV) (definition)
    Average volume of erythrocytesin a specimen, elevated or decreased in accordance with average erythrocytesize;
  35. Mean corpuscular (RBC) volume (MCV) normal range:
    Adult~80-96 femtoliters(fl) / RBC
  36. A group of disorders characterized by changes in the structure of hemoglobin, can also cause a low MCH level.
  37. Mean corpuscular hemoglobin (MCH) (definition)
    the amount of hemoglobin in an average erythrocyte.
  38. Mean corpuscular hemoglobin (MCH) (calculation)
    Calculated by multiplying the total amount of hemoglobin by .001 and dividing that number by the total number of red blood cells/.001.
  39. Mean corpuscular Hemoglobin normal range-
    between 26 and 33 picogramsof hemoglobin per red blood cell
Card Set
BIOL 2510 Blood Lab
Lab Practical #1 blood lab