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Cells:
smallest structural unit of living matter functioning independently
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Extracellular matrix:
- mesh-like material that holds cells and tissues together
- Made of gel-like substance and fibers
- active structure of molecules secreted by cells that provides structural and biochemical support to surrounding
- a component of all living tissues.
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Epithelial Tissue-matrix is
scanty –thin mat- basal lamina –underlies epithelium
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What type of epithelium is in Skin and oral mucosa ?
- STRATIFIED SQUAMOUS EPITHELIUM
- Flat cells arranged in layers
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COMPOSITION of Epithelial Tissue
PLENTIFUL cells:
closely packed epithelial cells bound together in sheets - rest on connective tissue
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COMPOSITION of Epithelial Tissue
Sparse extracellular matrix:
small, thin mat under cellular sheets
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Keratinization
process by which epithelial cells on the surface of the skin become stronger and waterproof
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Keratinized epithelial cells
- no nuclei
- Most heavily keratinized epithelium of body is on palms of hands and soles of feet
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Nonkeratinized ephithelial cells:
- have nuclei
- Cushion mechanical stress and wear
- Softer -More flexible
- Mucosal lining of cheeks:
- Permits movement needed to speak, chew, and make expressions
- Non-keratinized surfaces must be kept moist by bodily secretions to prevent them from drying out
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Mucosal lining of cheeks
- Permits movement needed to speak, chew, and make expressions
- Non-keratinized surfaces must be kept moist by bodily secretions to prevent them from drying out
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Epithelial Cell Junctions:
- Cellular structures that mechanically attach a cell and its cytoskeleton to:
- Neighboring cells
- Basal lamina
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Purpose of Cell Junctions
- Allows cells to bind together -stronger structural unit
- SKIN-Epithelium that must withstand severe mechanical stresses have the most number of cell junctions
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Forms of Epithelial Cell Junctions
Desmosome:
- connects two neighboring epithelial cells
- Think of snaps on a jacket
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Forms of Epithelial Cell Junctions
Hemidesmosome:
connects epithelial cells to basal lamina
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Connective Tissue-
Fills spaces between tissues and body organs
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Extracellular matrix of connective tissue:
Main component in matrix is gel-like substance with a network of strong fibers
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Soarse cells:
CT CELLS-sparsely distributed in extracellular matrix
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Cells of Connective Tissue
FIBROBLASTS:
cells that synthesizes the extracellular matrix and collagen, is the structural framework (stroma) for tissues, and plays a critical role in wound healing.
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Cells of Connective Tissue
MACROPHAGES AND NEUTROPHILS:
- Phagocytes (Cell Eaters-PAC MAN)
- devour dying cells- invasive microorganisms
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Cells of Connective Tissue
Lymphocytes (WBC):
Cells- immune system
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Connective Tissue
DENTAL connective TISSUES:
- CEMENTUM, DENTIN, PULP, ALVEOLAR BONE
- Enamel is epithelial tissue
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ameloblasts, which produce enamel become a part of the
reduced enamel epithelium
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Epithelial–Connective Tissue interface is
- the boundary where the epithelial and connective tissue meet.
- Wavy or smooth
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Wavy Boundary of Epithelial Connective Tissue Boundary includes:
- Epithelial ridges: Deep extensions of epithelium reach down into connective tissue
- Connective tissue papillae: Finger-like connective tissue extensions reach up to epithelium
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Function of WAVY Boundary
- Enhances adhesion of epithelial tissue and connective tissue by increasing surface area of junction between two tissues.
- Epithelium receive nourishment from CT.
- Epithelium doesn’t have it's own Blood Supply
- Strong junction allows skin to resist mechanical forces
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Gingival Epithelium:
- STRATIFIED SQUAMOUS EPITHELIUM
- Functions well in wet environment of oral cavity
- Anatomy is similar to that of skin
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Three Anatomic Areas of the Gingival Epithelium?
1.Oral epithelium
2.Sulcular epithelium
3.Junctional epithelium
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Oral Epithelium:
- Covers outer surface- free gingiva/ attached gingiva
- Located from the Crest of gingival margin to mucogingival junction
- Keratinized/ Parakertinized(partially keratinized)
- Interface-connective tissue- Wavy
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Sulcular Epithelium:
- Epithelial lining of gingival sulcus
- Extends from crest of gingival margin to coronal edge of junctional epithelium
- Thin and nonkeratinized
- Permeable Fluid flows from connective tissue to sulcus
- Smooth interface with connective tissue
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Junctional Epithelium
- Forms the base of sulcus
- Joins gingiva to tooth
- More Cells Apical than coronal
- 15 to 30 cells thick in coronal zone
- Four to five cells thick in apical zone
- Thin and nonkeratinized
- Easiest point of entry for bacteria to invade connective tissue
- Smooth tissue interface with connective tissue
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Internal basal lamina
Thin basal lamina Between epithelial cells of junctional epithelium and tooth
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External basal lamina
Thin basal lamina Between epithelial cells of junctional epithelium and gingival connective tissue
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▪Dental pellicle
- Forms immediately on a clean tooth surface
- Protective by diffusing acid
- Enables microbial attachment in early stages of biofilm formation
- Lies between tooth and internal basal lamina
- Also known as dental cuticle or acquired pellicle
- Forms during late stages of tooth eruption
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JE Attachment to Tooth
- Attaches to tooth surface using hemidesmosomes and internal basal lamina
- Cells of the junctional epithelium can move along the tooth surface
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Attachment to Underlying Gingival Connective Tissue
- Attaches using hemidesmosomes and external basal lamina
- Healthy junctional epithelium has a smooth tissue interface with connective tissue
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Function of Junctional Epithelium–Connective Tissue Interface
- Provides a seal at base of gingival sulcus or periodontal pocket
- Provides a protective barrier between plaque and connective tissue
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Function of Gingival Connective Tissue
- Provides solidity to gingiva
- Attaches gingiva to cementum of root and bone
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Components of Gingival Connective Tissue
- Few cells (5%):
- Fibroblasts, neutrophils, macrophages, lymphocytes
- Abundant extracellular matrix:
- 55% to 65% protein fibers (collagen)
- 30% to 35% gel-like matrix
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Supragingival Fiber Bundles
Rope-like collagen fiber bundles located coronal to alveolar crest
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Characteristics of Fiber Bundles
- Embedded in extracellular matrix
- Junctional epithelium attachment to tooth is strengthened by fiber bundles
- Provides structural support to gingival tissues
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Function of Fiber Bundles
- Brace free gingiva firmly against tooth
- Reinforce attachment of junctional epithelium to tooth
- Provide free gingiva rigidity to withstand chewing
- Unite free gingiva with cementum of root and boneConnect adjacent teeth to each other
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Classification of Fiber Groups
- Alveologingival
- Circular
- Dentogingival
- Periostogingival
- Intergingival
- Intercircular
- Interpapillary
- Transgingival
- Transeptal
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