Biochem Translation Antibiotics and Misc Stuff

  1. binds to the 30S subunit (prokaryotic) and distorts its structure interfering with the imitation of protein synthesis
  2. bind to the 30S subunit (prokaryotic), blocking access of the aminoacyl-tRNA to the mRNA-ribosomal complex
  3. bears a structural resemblance to aminoacyl tRNA and becomes incorporated into the peptide chain causing inhibition of further elongation in both prokaryotes and eukaryotes
  4. inhibits prokaryotic peptidyltransferase and high level also inhibit mitochondrial protein synthesis
  5. bind irreversibly to a site on the 50S subunit on the bacterial ribosome, thus inhibiting translocation
    Clindamycin and Erythromycin
  6. inactivates the eukaryotic elongation factor eEF-2 thus preventing translocation
    Diphtheria toxin
  7. major form of chromsomal DNA
    Right handed helix
    10 base pairs per 360* turn
    B form of DNA
  8. found in DNA-RNA (hybrid) and in double stranded RNA
    formed by moderate dehydration of B form
    right handed helix
    11 base pairs per 360* turn
    A form of DNA
  9. Found in regions of DNA that contain alternating purines and pyrimidines 
    the deoxyribose phosphate backbone zig zags 
    It is a left handed helix
    contains 12 BP per turn
    Z form of DNA
  10. Polymerase that transcribes rRNA genes in the nucleolus
    RNA Polymerase I
  11. polymerase that transcribes mRNA genes
    RNA polymerase II
  12. RNA polymerase that transcribes tRNA genes
    RNA polymerase III
  13. what allows 30 species of tRNA to bind to 61 different codons
    the wobble
  14. in the wobble

    G -->
    • C and U
    • A and G
    • U, C, and A
    • G
  15. What are the three important differences between prokaryotic and eukaryotic translation?
    • 1. in prokaryotes transcription and translation are closely linked (can be like one right after the other or at the same time i think)
    • 2. prokaryotes have a SHINE-DALGARNO sequence 6-10 bases upstream from the initiating start codon (THIS IS NOT A PROMOTER, but sends a message to ribosome that AUG is coming)
    • 3. Prokaryotic mRNA is often polycistronic, while eukaryotic mRNA is NOT
  16. what makes the peptide bone between AA in translation?
    peptidyl transferase

    a ribozyme
  17. Vitamin K can act as an enzyme in which post-translational modification?
    carboxylation of Glutamate to form gamma-carboxyglutamate
Card Set
Biochem Translation Antibiotics and Misc Stuff
Material for the Final Exam