Serological Problem Solving for Antibodies to high incidence antigens

  1. Clues in phenotypes
    • Recently transfused?
    • S-s- may be U-
    • P1 may be P- or Vel-
    • M-N- may be Ena-
    • Negative for both common alleles may be have a null phenotype
    • Group O may be H-
  2. Problem solving options
    • Test selected cells negative for high incidence Ag's.
    • Inhibition or neutralization studies
    • Adsorption and elution to separate antibodies
    • Chemical or enzyme treatments
  3. Clues in phenotypes
    • Vel- are also sometimes P1-
    • RBCs Ena- or MkMk phenotypes (which lack glycophorin A type as Wr(a-b-)
  4. Only positive with DTT/AET
    • Bpa
    • Ch/Rg
    • Xg
  5. Negative for all chemicals & enzymes
    • IN
    • JMH
  6. Positive with DTT/AET & a-chymotrypsin
    • M
    • N
    • ENaTS
    • Ge2 &Ge4
  7. Positive with DTT/AET & Trypsin
    *Variable with Ficin/papain
    S & s
  8. Positive with Trypsin
    Variable with DTT/AET
    YT
  9. Positive with DTT/AET, a-chymotrypsin, & trypsin
    EnaFS
  10. Positive with Ficin/papain
    Variable with DTT/AET
    • LU
    • MER2
  11. Positive with Papain
    Variable with Ficin
    KN
  12. Positive with Ficin/Papain
    Weak with a-chymotrypsin
    DO
  13. Positive with Ficin/Papain, Trypsin
    Weak with DTT/AET
    CROM
  14. Positive with ficin/papain, trypsin, & DTT/AET
    Some DI
  15. Positive with ficin/papain, trypsin, &
    Pos or weak with a-chymotrypsin
    LW
  16. Positive with all 
    May be weak with Trypsin and a-chymotrypsin
    SC
  17. Positive with Trypsin, papain/ficin, a-chymotrypsin
    • Kel
    • *Except KALT which is destroyed by trypsin
  18. Positive for all
    • ABO,
    • AnWj, Sda
    • ENaFR, U, P1PK, RH, LE, FY3, JK,
    • most DI, CO,
    • H, Ge3, OK, I/I,
    • P, FORS, JR, LAN, CSa, ER, LKE, PX2, Vel, ABTI, Ata, EMM,  PEL,MAM
  19. Positive for all with * Enhanced activity with DTT/AET
    Kx
  20. Most common in whites
    • k
    • Era
    • Lan
    • Kpb
  21. Black common antibody marker
    • U
    • Jsb
    • Joa
    • Cra
    • Esa
    • Ata
  22. Japanese common antibody marker
    • UMC
    • Jra
    • Oka
    • Lan
  23. HTLA
    *Obsolete name*
    High Titer Low Avidity Antibodies
  24. Do not use HTLA anymore, however others may, so be familiar with those that have those characteristics
    • Chido/Rodgers
    • Knops
    • Dombrock
    • Cost
    • John Milton Hagen
Author
Kwalke12
ID
335869
Card Set
Serological Problem Solving for Antibodies to high incidence antigens
Description
Serological Problem Solving for Antibodies to high incidence antigens
Updated