protein procedure 2

initial biuret used for measurement of urine protein

folin-lowry test
principle of folin-lowry test

oxidation of: tyrosine, tryptophan, and histidine by residues of folin-phenol reagent
based on most ability of most proteins in serum to bind dyes

dye binding
example of dyes:
- amido black 10B
- bromphenol blue
- bromcresol purple
- bromcresol green
- coomasie brillant blue
- HABA
- Lissamine green
- Methyl orange
- Ponceau S
used to measure protein concentration in CSF or urine

turbidimetric method
turbidimetric
protein forms precipitate in addition of?
- trichloroacetic acid
- sulfosalycilic acid
turbidity produced caN BE MEASURED BY

optical density
not specified to proteins because nucleic acids can also precipitate

turbidimetric
easiest means of separating proteins- based on the changes in the solubility of protein , (charge ion and dehydrating solvent)

salt fractionation : precipitation
globulins are precipitated using either
- sodium sulphate
- sodium sulphite
- ammonium sulphate
- methanol

Author

Anonymous

335695

Card Set

protein procedure 2

Description

midterms

Updated

2017-11-05T08:23:33Z