Coagulation / Hemostasis Lab Tests

  1. TT
    Thrombin Time
    Measures the ability of thrombin to convert fibrinogen to fibrin and is useful in the evaluation of circulating antioagulants (pathologic inhibitors)
  2. TT is prolonged in what siutations
    • Hypo & Dys fibrinogenemia
    • Patients on heparin therapy
    • Circulating FDP's
    • Pathologic circulating inhibitors
  3. Mixing Studies
    Patient plasma is mixed with normal plasma in varying concentrations, then incubated for 2 hours.

    Corrected = Deficiency
  4. Reptilase Time
    • Similar to TT, except it uses reptilase instead of thrombin.
    • Not affected by heparin

    Prolonged with: A & Dys fibrinogenemia
  5. Russell's Viper Venom
    Test is based on the premise that LA/aPL activity is enhanced in the presence of reduced phospholipids. The reagent contains dilute RVV, CaCl, & phospholipids. The reagent is added to PPP. RVV activates FX resulting in a clot formation.


    If Las/aPLs are present, the patients dRVVT is longer than that of the normal control
  6. List inherited coag disorders
    • vWD
    • TTP
    • FV Leiden
    • Hemophilia
    • Bernard-Soulier
    • Glanazmanns Thrombasthenia
  7. List acquired coag disorders
    • vWD
    • ITP
    • DIC
  8. Coumadin affect which coag factors
    • 2
    • 7
    • 9
    • 10
  9. Heparin inactivates which coag factors
    • Thrombin
    • 9
    • 10
    • 11
    • 12
  10. What is the purpose of mixing studies
    To identify a factor deficiency
  11. What coag factors are missing in aged serum
    • 1
    • 2
    • 5
    • 8
    • 13
  12. What is 5M Urea test useful for
    It can qualitatively if a patient is deficient in FXIII by obersving dissolution of a clot after 24 hours of incubation
  13. What factor deficiency is present given a PT of 15 seconds, a PTT of 60 seconds, a mixing study using aged serum and absorbed plasma that corrected both in the case of the former but corrected neither in the case of the latter?
    F 10 is likely deficient: F10 is present in aged serum and is in the common pathway so that both the PT and the PTT should be affected. F10 is not present in adsorbed plasma
  14. Extrinsic Pathway Coag Factor
    Factor 7
  15. Intrinsic Pathway factors are:
    • 8
    • 9
    • 11
    • 12
  16. Common Pathway Factors are:
    • 1
    • 2
    • 5
    • 10
  17. What is the normal range for: PT
    11-13 seconds
  18. What is the normal range for: aPTT
    22-32 seconds
  19. List congenital disorders that affect the NUMBER of platelets
    • Bernard-Soulier
    • May-Hegglin anomaly
    • TAR syndrome
  20. Increased destruction of platelets may be caused by:
    • ITP (post viral illness in children, spontaneous in adults)
    • Posttransfusion purpura (1week post, antibodies against P1A1 are produced) - Treat with plasmaphoresis without exchange and gamma globulin
    • Isoimmune Neonatal Tfhrombocytopenia (antibodies against  baby's plateelts)
    • Drug induced ITP (Quinine or Heparin)
  21. vWd Type 1 lab results
    Decreased: vWF Ag, RIPA, & F8

    PTT might be prolonged
  22. vWD Type 2a lab results:
    Decreased RIPA

    Normal Factor 8 and PTT
  23. vWD Tfype 2B lab results:
    • Increased RIPA
    • Decreased Platelets

    Normal Factor 8 & PTT
  24. vWF Type 2N lab results
    Decreased Factor 8

    Normal; vWf: Ag, R:Co
  25. vWD Type 3 lab results
    • Increased PTT & PFA
    • Decreased: vWF Ag
    • Absent: RIPA
  26. Glanzmann's Thrombasthenia lab results
    • Decreased: GPIIb/IIIa
    • Increased: BT & PFA
    • Abnormal: ADP aggregation, thrombin, collagen, & epinephrine
  27. Bernard-Soulier lab results
    • Decreased: Plts, GPIb
    • Increased: BT & PFA
    • Abnormal: thrombin aggregation
  28. ITP Lab Results
    Severe Thrombocytopenia: <20K
  29. Afibrinogenemia lab results
    Increased: BT, PT, PTT, TT, RT

    Absent: Fibrinogen
  30. Dysfibrinogenemia lab results
    Increased: TT, RT, 

    Normal: Fibrinogen

    *may have prolonged PT & PTT
  31. Hypofibrinogenemia lab results
    • Increased: TT
    • Decreased: Fibrinogen

    Normal: PT & aPTT
  32. PT Measures what pathway and is prolonged with what deficiencies
    Extrinsic Pathway

    1,2,5,7,9,& 10
  33. PTT measures what pathway and is prolonged with what deficiencies
    Intrinsic Pathway

    2,5,8,9,10,11,12 & fibrinogen (1).
Author
Kwalke12
ID
335324
Card Set
Coagulation / Hemostasis Lab Tests
Description
Coagulation related lab tests
Updated