-
Thylakoid
Membrane that contains chlorophyll
-
What's inside a chloroplast?
- Double membrane (outer/inner)
- Inermembrane space
- Stroma
- Thylakoid
- Thylakoid space
-
Photosynthesis formula
- 6 Water + 6 carbon dioxide + sunlight ->
- Glucose + 6 oxygen
-
History of photosynthesis formula
- O2 originally thought to come from the CO2
- van Niel first proposed that O2 came from H2O in 1930s
- Confirmed in 1950s
-
How is photosynthesis a redox reaction
- Water becomes oxidized to become oxygen
- Carbon dioxide is reduced to become sugar
-
Is photosynthesis endergonic or exergonic?
Electrons increase in potential energy so the reaction is endergonic
-
What form of energy is light?
A form of electromagnetic radiation
-
Photons
discrete particles with a fixed amount of energy (no mass!)
-
Why is the sky blue? Why does something appear white? black?
- This deals with how much wavelengths are making it to your eyes
- Black absorbs all visible light
- White reflects all visible light
-
Pigment
- Substance that absorbs visible light
- Different pigments absorb different wavelengths of light
-
Why do chlorophyll a & b appear green?
They do not absorb wavelengths in that part of the spectrum
-
Why do leaves change colors?
Leaves stop producing chlorophyll so the other chemicals start showing through
-
Why do carotenoids exist?
To protect the plant. The pigments pick up the extra energy from the sun
-
Why does chlorophyll appear green
It reflects green wavelengths
-
Two parts of photosynthesis
- Light reactions
- Calvin cycle
-
Light reaction
- Converts light energy into chemical energy
- Located in the thylakoid membrane.
- Light + H2O -> NADPH + ATP + O2
- H2O is split to provide e- and H+
- NADP+ is reduced to NADPH
- ATP is produced
- O2 is given off
-
Antenna complexes
Contain pigment molecules which absorb light and transfer the energy to reaction-center complex (i.e light harvesting complex)
-
Reaction-center complex
Contains a special pair of chlorophyll a molecules which can reduce the primary electron acceptor
-
What helps direct energy to the center in the antenna complex?
- Resonance energy transfer
- Energy in electron is transferred to nearby pigment
-
In the thylakoid membranes, what is the main role of the pigment molecules in a light-harvesting complex
Transfer light energy
-
As electron pass through the system of electron carriers after leaving photosystem II, they lose energy. What happens to the energy?
It is used to establish and maintain a protein gradient for the generation of ATP
-
Photophosphorylation
The process of making ATP from ATP synthase. This is because the energy is coming from photons
-
When oxygen is released as a result of photosynthesis, it is a direct by product of?
Splitting water molecules
-
Cyclic electron flow
- Electrons from photosystem I transferred back to ETC
- Produces ATP instead of NAPDH
-
How are the light reactions and the Calvin Cycle connected?
The light reactions provide ATP and NADPH to the Calvin Cycle, and the Calvin cycle returns ADP, Pi and NADP+ to the light reactions
-
Calvin Cycle
Uses the chemical energy stored in ATP and NADPH to reduce CO2 to sugar (G3P)
-
3 Phases of Calvin Cycle
- Carbon fixation
- Reduction
- Regeneration of CO2 acceptor
-
Carbon Fixation
- First phase in Calvin Cycle
- Rubisco (RuBP carboxylase) attatches to CO2 molecule to RuBP
- Taking CO2 and fixing it to become an organic molecule
-
Reduction
- Second phase in Calvin Cycle
- Carbon intermediate phosphorylated by ATP
- Reduction by NADPH and loses the phosphate = G3P
-
Regeneration of CO2 acceptor
- Series of steps which utilize ATP to rearrange 5 molecules of G3P to regenerate 3 molecules of RuBP
- Majority of sugar produced is turned into RuBP
-
What process is most directly driven by light energy
Removal of electrons from chlorophyll molecules
-
What does it mean when CO2 becomes fixed during photosynthesis?
CO2 becomes bonded to an organic compound
-
How many times must the Calvin Cycle occur to produce 1 net G3P
- 3 times
- For 1 G3p, cycle uses 9 ATP and 6 NADPH
-
Which doesn't happen in Calvin Cycle?
Carbon fixation
Oxidation of NADPH
Regeneration of CO2 acceptor
Consumption of ATP
Release of oxygen
Release of oxygen
-
Photorespiration
- When Rubisco fixes oxygen in place of carbon dioxide.
- This causes plants to release carbon dioxide
- It also consumes ATP and decreases photosynthesis by siphoning off material from the Calvin Cycle
-
When is trouble with Rubisco most prevalent and why?
- Hot, dry climates
- Plants close stomata to minimize water loss but this reduces the amount of carbon dioxide available
- Concentration of oxygen released from light reactions increases favoring oxygen fixation
-
C3 plants
Plants that use rubisco for initial carbon fixation
-
Plants that have alternative modes of carbon fixation to minimize photorespiration
- C4 Plants
- CAM (crassulacean acid metabolism)
-
What happens in C4 plants?
- Carbon fixation is in a different location from the Calvin Cycle
- CO2 is initially fixed by PEP carboxylase in mesophyll cells to form a 4-carbon product
- Product is transported into bundle sheath cells where the CO2 is released and can be added to the Calvin Cycle by rubisco
- This process uses some energy but it helps ensure that the rubisco fixes only CO2
-
CAM Plants
- Carbon fixation is separated from the Calvin Cycle at a different time
- Plants close their stomata during the day to conserve water and open at night
- Plants take in CO2 and fix it into organic acids which are stored in vacuoles
- During the day, when light can supply ATP and NADPH, CO2 is released from the organic acids and enters the cycle
- This also uses some energy
-
The alternative pathways of photosynthesis using the C4 or CAM systems are said to be compromises why?
Both minimize photorespiration but expend more ATP during carbon fixation
-
What happens to the sugar produced?
- 50% is used for cellular respiration
- Linked together to form cellulose -> build up the cell wall
- Excess stored as starch in the winter for trees
-
If you plant a maple seed in your backyard and over the course of many years it grows into a tall tree, where did the increase in mass come from?
- Air
- Carbon is pulled out of the air which gets bonded to organic molecules and turned into cellulose
|
|