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required for the preparation or primary standards
analytical balance
mechanical balance aka
substitution balance
types of centrifuges
horizontal or swing bucket
fixed angle or angle head
ultracentrifuges
(Type centrifuge?)
allows the tubes to attain in a horizontal position when spinning and a vertical position when the head is not moving
horizontal or swing bucket
(Type centrifuge?)
horizontal or swing bucket can attain speeds of up to approximately
3000 rpm
(Type centrifuge?)
horizontal or swing bucket
(?rpm)
excessive heat build up due to increased _______
3000rpm
air friction
(Type centrifuge?)
have angled compartments for the tubes and allow small particles to sediment more rapidly
fixed-angle or angle head
(Type centrifuge?)
(fixed-angle or angle head) angle is fixed at around ___
52 degrees
(Type centrifuge?)
high-speed centrifuges used to separate layers of different specific gravities
ultracentrifuges
(Type centrifuge?)
they are commonly used to separate layers of different specific gravities
ultracentrifuges
(Type centrifuge?)
chamber is refrigirated to counter heat through friction
ultracentrifuges
(Type centrifuge?)
used to check the speed of the centrifuge
tachometer
tachometer aka
strobe light
a process of ensuring accuracy and precision in the laboratory by including quality control reagents in every series of measurement
quality control
process of monitoring laboratory analyses to ensure accuracy of results
quality control
measure of central tendency
mean
midpoint of a distribution
median
most frequent observation
mode
difference between the highest and lowest values
range
a measurement of dispersion
standard deviation
sd2
variance
an index of precision used to compare the dispersion of two or more groups of data with different units
coefficient of variation
% sodium citrate used in light blue
3.2%
ratio of sodium citrate in light blue
1:9
% sodium citrate used in black top (ESR)
3.8%
ratio of sodium citrate in black top (ESR)
1:4
red top - ___________ standing before centrifugation
15 to 30 min
action of EDTA
chelates calcium
use for STAT (emergency)
yellow/gray and orange
STAT tubes stand for _____ before centrifugation
5 to 15 min
reagent grade...
high levels of purity
Ultrapure
reagent grade...
safe
United States Pharmacopeia
most preferred h20
type 1
produced by distilled h20
then cationized/anionized
type 1
-glassware?
-ideal for acidic solution
pyrex (borosilicate)
-glassware?
-resist heat, thermal shock and corrosion
pyrex (borosilicate)
-glassware?
-ideal for high precision analytical work
high silica
-glassware?
-ignition techniques
vycor
-glassware?
-handling heat labile substances _________
low actinic (amber colored)
300-500
-glassware?
-not reliable
flint (soda lime)
-glassware?
-lowest cost
flint (soda lime)
-glassware?
- disposable
flint (soda lime)
has 2 edge rings
blowout
-relies on gravity
-one ring
self-draining
(pipette)
highest accuracy
volumetric
(type centrifuge?)
-recommended for separator tubes
horizontal or swing bucket
(type centrifuge?)
-not recommended for separator tubes
fixed-angle or angle head
(type centrifuge?)
-reference method for lipoprotein analysis
ultracentrifuge
Author
summerdhon
ID
334053
Card Set
specimen collection
Description
cc1
Updated
2017-09-08T10:42:46Z
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