what are three examples of solid phase automations in the blood bank?
ABS2000
ROSYS plato
The Galileo
this automated technique performs ABO/RH and AB screens/crossmatch using a gel card containing dextran acrylamide gel particles, and does not require washing.
the gel system
what are two examples of the gel system?
ortho provue (fully automated)
tecan megaflex (semi automated)
Lukemia depressens _______ expression on the RBC.
antigen
what is an example of depressed antigen expression?
acquired B phenomenon
Carcinomas of the ______ or _______ can cause antigens to be undetectable.
stomach
pancreas
what are three diseases that can cause acqured B phenomenon?
intestinal obstruction
Carcinoma of colon or rectum
Septicemia with E. coli or Proteus mirablis
various lymphoma's, malignant non-hodgkin's, very youg or elderly patients, or immunosuppressant therapy can cause a ________ production of ABO ISO-antibodies.
Decreased
true or false
discrepancies between forward and reverse grouping are generally technical in nature.
true
what are three causes of tecnical error in discrepancies between forward and reverse typing?
recheck ID of specimen
Clerical errors
Failure to add reagent.
if not technical, ABO discrepancies fall into one of ______ major categories.
four
which group of ABO discrepancies involves antibodies.
group I
which group of ABO discrepancies involves antigen?
Group II
which group of ABO discrepancies involves protein?
group III
which group of ABO discrepancies involves miscellaneous?
group IV
what are three causes of group I discrepancies?
immunodeficiency
leukemia or lymphoma
chimerism
this is a rare condtion in which there are two cell population in an individual.
chimerism
true or false
chimerism is often artificial?
chimerism
normal ABO reactions are ___ or stronger?
3+
if reactions are 1+ or less: incubate for 15-30 minutes at _____ temp.
room
what are three causes of group II discrepancies?
minor subgroups of A or B
Hodgkin's disease
Acquired B phenomenon
which group of discrepancies can be caused by group specific substances?
group II
in group III discrepancies, __________ formation can cause pseudo agglutination.
rouleaux
multiple myeloma and hodgkin's lymphomas can cause what group of discrepancies?
group III
_________ the patient's red cells several additional times with saline will remove most of the protein causing the rouleaux formation.
washing
the resolution of which group of descrpancies involves saline replacement techniques?
group III
what are five causes of group IV discrepancies?
polyagglutination (T antigen)
Cold reactive antibodies
warm autoantibodies
unexpected ABO iso-antibodies
Antibodies against the antisera
for group IV discrepancies patients cells should be tested with what?
lectin
the resolution of what group of discrepancies may involve warming the blood to 37oC?
Group IV
this is the removing of antibody from surface of RBC.
elution
the resolution of which group of discrepancies may involve elution?
group IV
True or false
all ABO discrepancies must be resolved before blood is released to patient for transfusion.
true
all reagents for ABO typing should be stored at what temperature when not in use?