A&_P_Lab microscope.txt

Power switch, Base, fine adjustment, coarse adjustment, light control, arm, head, ocular lenses, rotating nose piece, objective lenses, stage, mechanical stage, iris diaphragm lever, condenser, substage light

supports the microscope

located in the base, light passes directly upward through the microscope

platform the slide rests on while being viewed- mechanical stage and/ or stage clips hold the slide in place

small substage lens that concentrates light on the specimen- may have a rack & pinion knob that raises and lowers the condenser to vary light delivery.

arm attached to the base of the condenser that regulates the amount of light passing through the condenser, permits the best possible contrast for viewing

used to focus on the specimen

used for precise focusing once the coarse focus is accomplished

supports the objective lense system (mounted on the nosepiece) and ocular lenses

vertical portion of the microscope that connects the base and head

1 or 2 lenses at the superior end of th head. 10X magnification also has a pointerin one ocular lens

rotating mechanism at the base of the head, carries 3 or 4 objective lensesover te light beam passing through the hole in the stage

scanning, 4 X or 5X used for scanning the slide it is the shortest lense; low power, typically 10X, high power, ranges from 40X to 50X

image projected to the ocular

the real image that is magnified by the ocular lens

magnification of any specimen being viewed is equal to the power of the ocular lens multiplied by the power of the objective lens used.

ex: ocular lens 10X and objective lens 45X total magnification is 450X (10 x 45)

ability to discriminate two close objects as seperate

determined byt he amount and physical properties of the visible light that enters the microscope

the distance from the objective lens to the specimen

the area you see through the microscope

• *hold upright with one hand on the arm and one on the base
• *grit-free lens paper to clean, in a circular motion
• *begin focusing on the lowest power objective lens
• *use coarse adjustment knob only with the lowest power lens
• *remove slide, rotate to lowest power lens, wrap cord, replace dust cover for storage
• *never remove any parts from the microscope

images viewed on the microscope are reversed, the top of the specimen becomes the bottom and right becomes left

fine focus will reveal depth of focus (layers top, middle, bottom)