histopath-instrument

most common type of microscope used in histopathology laboratory

In relation to hematological studies and for elements with low refractive indices like

specimens seen under PHASE CONTRAST microscope

fats and crystals

Produces 3D image

For naturally fluorescent microorganisms or those stained by fluorescent dyes/fluorochrome.

PARTS OF MICROTOME

(part of microtome)
where the tissue is held in position.

(part of microtome) for actual cutting of tissue sections

(part of microtome) line up the tissue block in proper position with knife, adjusting the proper thickness of the tissue for successive sections.

TYPES (KINDS)OF MICROTOME

TYPES (KINDS)OF MICROTOME
for cutting of serial sections of large blocks of paraffin embedded tissues.

for cutting paraffin embedded sections.

for cutting celloidin embedded sections.

for cutting unembedded sections.

for cutting sections for EM

is an apparatus used in fresh tissue section microtomy, consisting of an isolated microtome housed in an electrically driven refrigerator chamber, maintained at temperature near

automated tissue processor which fixes, dehydrates, clears and infiltrates tissues for a more rapid diagnosis.

FRESH TISSUE EXAMINATION

USEFUL TECHNIQUES IN CYTOLOGICAL EXAMINATION

tissue is immersed in a watch glass containing isotonic salt sol’n and examined under phase contrast or bright field microscope.

tissues <1mm in diameter are placed in microscopic slide and forcibly compressed with another slide/coverglass.

cellular materials are spread lightly over a slide by means of a wire loop or applicator, or by making an apposition smear with another slide.

by the use of applicator stick

material is transferred to a clean slide. (for smear preparations)

.......... technique of smear preparation has the advantage of maintaining the cellular interrelationship of the material to be examined.

............ smear has an added advantage in that cells maybe examined without destroying their actual intercellular relationship, and without separating them from their normal surroundings.