proteins

PROTEIN
elements

PROTEIN
Have high molecular weight ......

PROTEIN
Made up of ..... joined by .....

CHON
Made up of an..... and a .....
Amphoteric
Assume .... at ....

CHON
Functions

Transportation of metabolic substances

Protein present in highest concentration in the serum

binds copper; is a ferroxidase

carries oxygen through our body, gives blood its red color

Transport ions

Most plasma proteins are synthesized in the ...

CHON is Secreted by ...  into the circulation 
except ...... - synthesized in ...... )

Intracellular proteins are made by.... while proteins synthesized in ...  are made by

Hormones that assist protein synthesis (4)

Hormones that assist controlling protein catabolism

Classification of proteins according to function

Classification of proteins according to function:
catalyze biochemical reactions

Classification of proteins according to function:
chemical messengers that stimulate or prevent actions of specific cells or organs

Classification of proteins according to function:
carry molecules transferrin ( iron ) HDL, LDL
(cholesterol, other lipids )

Classification of proteins according to function:
neutralize foreign materials

Classification of proteins according to function:
reserves of metal ions and amino acids

Classification of proteins according to function:
fibrous molecule
Provide cellular or body support collagen
(bone, skin )

Laboratory tests/Total protein methods

most frequently analyzed protein

PLASMA PROTEINS 2 GROUPS

ALBUMIN main function is

Pre-Albumin is not visible upon ....

Globulin
-Class of simple proteins ( as myosin )
-Insoluble in .... but soluble in ....

lab test/total protein methods:
Consist of an acid digestion of proteins to release ammonium ions nitrogen containing compounds.

lab test/total protein methods:
PRINCIPLE: Digestion of protein: measurement of nitrogen content

lab test/total protein methods:
under colorimetric method

lab test/total protein methods:
Depends on the presence of two or more peptide bonds which form a Purple complex with copper salts in alkaline solution.

lab test/total protein methods:
Most used

lab test/total protein methods:
PRINCIPLE: Formation of violet-colored chelate between Cu2+ ions and peptide bonds

lab test/total protein methods:
Involves oxidation of phenolic compounds such as Tyrosine, Tryptophan and Histidine to give a  Deep blue color.

lab test/total protein methods:
Initial biuret test used for measurement of Urine protein

lab test/total protein methods:
PRINCIPLE: Oxidation of tyrosine, tryptophan, and histidine residues by Folin-phenol reagent -Measurement of resultant Blue Color

lab test/total protein methods:
PRINCIPLE: Protein binds to dye and causes a spectral shift in the absorbance maximum of the dye

lab test/total protein methods:
-Used to measure protein concentration in CSF or Urine

Turbidity can be measured by

IDENTIFICATION AND QUANTIFICATION OF PROTEINS

types of column separation

IDENTIFICATION AND QUANTIFICATION OF PROTEINS
method?
Globulins are precipitated using either ....  or  ...... leaving albumin in solution.

IDENTIFICATION AND QUANTIFICATION OF PROTEINS
separated proteins on the basis of their electric charge

for each protein, there is a pH where the net charge on molecule is zero. This value is called ....... for that protein.

At pH values greater than isoelectric point, the net charge on the protein is ...... .
At lower pH values, the protein has a .........

IDENTIFICATION AND QUANTIFICATION OF PROTEINS
samples are applied  at high salt and eluted with low salt.

IDENTIFICATION AND QUANTIFICATION OF PROTEINS
Based on specific binding between protein of interest and protein that has been convalently linked to the solid support medium of a column.

Separation method based on flow through a capillary tube that can be tailored to resolution of different molecules based on size, hydrophobocity, or stereospecificity