PrevMed3- Food Safety Cases

• diarrhea (bloody)
• severe stomach cramps
• vomiting
• +/- fever
• [in YOPI], hemolytic uremic syndrome

~72 hours

• Cattle- reservoir
• Sprouts, leafy greens- produce

adulterant (zero tolerance policy)

identify and investigate the majority of outbreaks

coordinated multi-staate outbreak investiagtions

• whole genome fingerprint
• restriction enzyme cuts chromosome intro fragments, and separation of fragments using pulsed field results in the "fingerprint"

isolation of a rare subtype at an unexpected frequency with pulsed field gel electrophoresis

• rapidly overtaking other techniques for strain differentiation
• more precise identification of outbreak-associated strains

Patient must seek medical attention--> doctor must make correct diagnosis and submit a sample--> clinical lab must recover the pathogen and send it to the public health lab--> the public health lab must subtype and isolate then report the results--> the health dept must investigate the outbreak

ingredient trace-back investigation for FDA regulated products

regulations classify E coli as an adulterant

• Diarrhea- bloody, mucousy
• Cramps
• Fever
• [usually self-limiting]

USDA FSIS (Salmonella is not considered an adulterant)