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MBio - Viruses

  1. Types of DNA Genomes (and examples)
    • Circular dsDNA - SV40
    • Linear dsDNA - T4 and/or T7
    • Linear ssDNA - Parvovirus
    • Linear dsRNA - Reovirus
    • Linear ssRNA - Poliovirus
  2. Viruses with multiple nucleic acid molecules
    • Reovirus (10 dsRNA)
    • Influenza (8ssRNA)
  3. 5-hydroxymethylcytosine
    • Unique base found in T-phage
    • Many viruses have unique bases
  4. Nucleocapsid
    Capsid + Genome
  5. Membrane envelope
    • Layer surrounding the viral capsid
    • Not found in all viruses
  6. Select viral taxonomic criteria
    • Cellular location of replication
    • sense of ssRNA strand (if applicable)
    • Number of capsomers (if applicable)
    • And more...
  7. Techniques for isolating viruses
    • Viruses are tough
    • Centrifugation (differential and density)
    • Precipitation - (NH4)2SO4 or PG
    • Denaturation of contaminants (Chloroform)
  8. Methods for counting viruses
    • Direct (With an EM) or indirect (below)
    • Hemaglutination assay
    • Plaque formation
    • LD50 (Animals)
  9. Perimissive cell
    Permits viral replication
  10. Outcomes of viral infection
    • Abortive - The virus is eliminated
    • Restrictive - Virus tends to stick around, quietly (but not silent)
    • Productive - Normal viruses infections, virions produced
  11. Ecplipse phase
    • Viral particles bound to the target cell
    • No longer infectious
  12. Stages of viral replication
    • Eclipse phase
    • Maturation
    • Latency (Both of the above combined)
  13. Two proteins involved in virus activation
    • Cro - Activates lysis if concentration is high enough
    • Lambda repressor (cI) - Causes lysogeny
  14. Pinocytosis
    Entry of an entire virion into an animal cell
  15. Viroid
    Virus with no DNA componenet
Author
Ant
ID
330242
Card Set
MBio - Viruses
Description
MBio
Updated

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