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Three characteristic properties of bacterial observation
Size, shape and association
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Three main bacteria shapes
Coccus, bacillus and spirillum
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Bacteria width (generally)
0.5-1um
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Bacteria length (generally)
1-5um
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Types of cell association
Single, pairs, clusters, chains
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Subsets of the chain arrangement
- Linear - Same plane of
- Palisade
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Factors affecting cell shape
- Culture condition
- Environment
- Temperature
- Age
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Most important dimension with regard to cell size
Cell length
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Ocular micrometer
- Scale on the microscope
- Arbritrary
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Some reasons cells are stained
- Determine reaction to stain (G+, G-)
- Determine info abt. cell wall (g. stain)
- Highlight metabolic processes
- Differentiate between living and dead cells
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How do chemical fixatives work
Cause proteinatious crosslinking
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Use of coomassie brilliant blue
Gel electrophoresis
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DAPI
- Nuclear stain
- Fluoresces blue
- Living or dead cells
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Eosin
- Stains erythrocytes, cytoplasmic material, cell membranes and extracellular structures
- Haemotoxylin counterstain
- Pink or red in colour
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