MBio Expt 1

Three characteristic properties of bacterial observation

Size, shape and association
Three main bacteria shapes

Coccus, bacillus and spirillum
Bacteria width (generally)

0.5-1um
Bacteria length (generally)

1-5um
Types of cell association

Single, pairs, clusters, chains
Subsets of the chain arrangement
- Linear - Same plane of
- Palisade
Factors affecting cell shape
- Culture condition
- Environment
- Temperature
- Age
Most important dimension with regard to cell size

Cell length
Ocular micrometer
- Scale on the microscope
- Arbritrary
Some reasons cells are stained
- Determine reaction to stain (G+, G-)
- Determine info abt. cell wall (g. stain)
- Highlight metabolic processes
- Differentiate between living and dead cells
How do chemical fixatives work

Cause proteinatious crosslinking
Use of coomassie brilliant blue

Gel electrophoresis
DAPI
- Nuclear stain
- Fluoresces blue
- Living or dead cells
Eosin
- Stains erythrocytes, cytoplasmic material, cell membranes and extracellular structures
- Haemotoxylin counterstain
- Pink or red in colour
Iodine
- Stain
- Starch

Author

Ant

330091

Card Set

MBio Expt 1

Description

MBio

Updated

2017-04-03T21:12:14Z

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