M06 - Enterobacteriaceae GNRs and Parasitic Bacteria

What is the optimal growth temperature and incubation conditions for Campylobacter jejuni?

A)  42C in microaerophillic and capnophilic conditions
B)  35C in microaerophillic conditions
C)  22C in capnophilic conditions
D)  35C in microaerophillic and capnophilic conditions
E) 42C in capnophilic conditions

A genital specimen was streaked on New York City medium and allowed to grow for 5 days at 37 C in 5% CO . Resulting colonies demonstrated a distinctive "fried-egg" appearance. What is the presumptive identity of this bacterium?

A)  Chlamydia trachomatis
B)  Treponema pallidum
C)  Mycoplasma hominis
D)  Rickettsia rickettsii

Choose the organism which is matched with the incorrect insect vector:

A)  Rickettsia ricketsii, ticks
B)  Orientia tsutsugamushi, mites
C)  Rickettsia typhi, fleas
D)  Coxiella burnetii, mites

How is Helicobacter pylori presumptively identified?

A)  A culture of a gastric biopsy
B) A rapid oxidase test on gastric scrapings
C)  A rapid urease test done on a gastric biopsy
D)  A culture of gastric scrapings

The primary method used to diagnose rickettsial diseases is

A)  serologic assays
B)  tissue culture
C)  testing the insect vector
D)  PCR

What would be the most appropriate screening tests to preemptively differentiate and identify the non-fermenting Gram negative bacilli from the Enterobacteriaceae?

A)  oxidase, indole, growth on BAP
B)  catalase, decarboxylation of arginine, growth on BAP
C)  motility, urease, morphology on MAC
D)  oxidase, TSI, nitrate reduction, growth on MAC

Rickettsiae that infect humans multiply preferentially within which of the following cells?:

A)  renal tubule
B)  red blood cells
C)  endothelial
D)  hepatic

Which media should be used to recover Bordetella pertussis from a nasopharyngeal specimen?

A)  MacConkey agar
B)  Bordet-Gengou agar
C)  Blood agar
D)  Chocolate agar

Which of the following products is responsible for satellite growth of Haemophilus spp around colonies of Staphylococcus aureus on blood agar plates?

A)  Tryptophan
B)  NAD (V factor)
C)  Hemin (X factor)
D)  Cystein

What is the selective agent in TCBS?

A)  saccharose
B)  Sodium thiosulfate & ferric citrate
C)  yeast extract
D)  oxgall & sodium cholate

Match the following diseases with their appropriate bacterial agent.

+Rickettsia rickettsii
+Ureaplasma urealyticum
+Rickettsia prowazekii
+Rickettsia typhi
+Mycoplasma pneumoniae
+Chlamydia psittaci
+Pseudomonas aeruginosa

-endemic typhus
-prostatitis, nongonococcal urethritis
-Parrot fever
-epidemic typhus
-swimmer's ear
-Rocky Mountain Spotted Fever
-"Walking pneumonia"

Which characteristic(s) apply to the HACEK bacteria?

A)  all of the above
B)  normal flora of oral cavity
C)  affinity for heart valves
D) capnophilic

Identify the bacterium based upon the following characteristics:
associated w/water environments
strict aerobe
ground-glass colonial morphology
requires L-cysteine for primary isolation
survives extreme ranges of environmental conditions for long periods of time

A)  Legionella pneumophila
B)  Kingella spp
C)  Aggregatibacter aphorophilus
D)  Helicobacter pylogenes

Match the following organism with it's morphological appearance or growth characteristic.

+drops of mercury on Regan-Lowe agar
+chlorine bleach odor
+grape-like odor
+rosettes and spindles
+fried-egg

-Eikenella corrodens
-Cardiobacterium hominis
-Bordetella pertussis
-Mycoplasma
-Pseudomonas aeruginosa

A 29-year-old man is seen for recurrence of a purulent urethral discharge 10 days after the successful treatment of culture-proven gonorrhea. The most likely explanation is the patient had a co-infection with:

A)  Chlamydia trachomatis
B)  Helicobacter pylori
C)  Uropathogenic E. coli
D)  Trichomonas vaginalis

Select the characteristic that does NOT apply to Helicobacter pylori.

A)  strong urease activity
B)  spiral-shaped
C)  transmission by aerosol
D)  50% adults over 60 infected

Which organism is described as being Gram negative gull-wing rods?

A)  Campylobacter jejuni
B) Helicobacter pylori
C)  Pseudomonas aeruginosa
D)  Haemophilus influenzae type b
E)  Vibrio cholera

Match the following characteristics with the appropriate bacterium

+growth around V factor disks
+localizes in tissues rich in erythritol
+darting motility
+requires X and V factors for growth
+causes soft chancroid disease

-Haemophilus influenzae
-Campylobacter spp.
-Haemophilus parainfluenzae
-Haemophilus ducreyi
-Brucella spp.

What organism may play a role in Guillain-Barré syndrome (GBS)?

A)  Helicobacter spp.
B)  Aeromonas spp.
C)  Campylobacter spp.
D)  Plesiomonas spp.

In order to speciate Vibrios recovered from a specimen, what media should be used?

A) CHOC
B) SBA
C) TCBS
D) BCYE

What antibiotic is primarily used to treat Rickettsial infections and Q fever?

A)  3rd generation cephalosporins
B)  Penicillin
C)  Tetracycline
D)  SXT

(T/F) Acinetobacter spp. may be mistaken for an enteric because it produces purplish colonies on MAC.
True False

This organism requires sodium for growth and can cause fatal septicemia in patients with an underlying liver disease.

A)  Vibrio vulnificus
B)  Vibrio cholerae
C)  Chromobacterium violaceum
D)  Aeromonas hydrophila

Plump Gram negative coccobacilli that tend to resist alcohol decolorization and may be mistaken for Gram positive cocci are

A)  Stenotrophomonas maltophilia
B)  Pseudomonas aeruginosa
C)  Bordetella spp.
D)  Acinetobacter spp.

Which best describes the source of Burkholderia cepacia infections

A)  All of the above are correct.
B)  Plants and soil
C)  Medical devices and medical solutions
D)  Water
E)  None of the above are correct.

The disease referred to as melioidosis is caused by

A)  Ralstonia pickettii.
B)  Burkholderia pseudomallei.
C)  Pseudomonas aeruginosa.
D)  Burkholderia mallei.

A 2-year-old patient is brought to his pediatrician with a purulent ear infection. The exudate is obtained and cultured. Based on the following growth characteristics, identify which organism was the cause of the ear infection.

Beta-hemolytic spreading colonies with a metallic sheen on BAP
Oxidase positive
Growth on MAC
Lactose negative
Growth at 42 C.
Corn-tortilla odor

A) Pseudomonas aeruginosa
B) Campylobacter jejuni
C) Aeromonas hydrophila
D) Bordetella pertussis

The Haemophilus influenzae vaccine protects against which serotype?

A)  Serotype b
B)  Serotype c
C)  Serotype a
D)  Serotype d

Which of the following diseases is most likely to be acquired from a hot tub or whirlpool?

A)  Pseudomonas dermatitis
B)  Q fever
C)  Acinetobacter cellulitis
D)  Erysipelas

An important characteristic of Chlamydia trachomatis is

A)  A Gram stain of the organism reveals Gram-negative bacilli
B)  Produces disease in humans and domestic animals
C)  Asymptomatic infections are common in females
D)  The bacteria survive long periods outside the host's body

Legionella pneumophila is the etiologic agent of both Legionnaires disease and

A)  Pontiac fever
B)  San Joaquin Valley fever
C)  Rift Valley fever
D)  Swine fever

Infection of the gastric mucosa leading to gastritis or peptic ulcers is most commonly associated with

A)  Helicobacter pylori
B)  Vibrio parahaemolyticus
C)  Campylobacter jejuni
D)  Salmonella Typhi

(T/F) Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide
True False

The optimal specimen for the recovery of Bordetella pertussis is

A)  Expectorated sputum
B)  Stool
C)  Nasopharyngeal swab
D)  Blood

(T/F) Stenotrophomonas maltophilia is oxidase positive
True False

All of the following are true regarding Coxiella burnetti except

A)  Found only in New Zealand
B)  Can replicate in the acidic environment of lysozomes
C)  Highly infectious
D)  Transmitted via inhalation of contaminated aerosols