(T/F) Low glucose levels, high protein and WBC counts in CSF is indicative of a bacterial meningitis.
True
Each species of gram positive cocci is associated with a particular disease or syndrome. Match each disease state with its most probable cause of infection. Answers can be used more than once.
+acute glomerulonephritis
+Pneumonia and meningitis in adults
+food poisoning
+dental caries
+rheumatic fever
+toxic shock syndrome
+Subacute bacterial endocarditis
+Honeymoon cystitis (bladder infection)
+Intra-abdominal abscess, sometimes resistant to vancomycin
+Pneumonia and meningitis in newborns
-Streptococcus pneumoniae
-Staphylococcus epidermidis
-Staphylococcus saprophyticus
-Group A Streptococcus
-Viridans streptococci
-Enterococcus
-Staphylococcus aureus
-Group B Streptococcus
acute glomerulonephritis: Group A Streptococcus
Pneumonia and meningitis in adults: Streptococcus pneumoniae
Intra-abdominal abscess, sometimes resistant to vancomycin: Enterococcus
Pneumonia and meningitis in newborns: Group B Streptococcus
Identify the species of gram negative diplococcus which ferments glucose and maltose.
A) Neisseria meiningitidis
What organism is associated with necrotizing fasciitis?
D) Group A Streptococcus
M. catarrhalis may be differentiated from the gonococci and meningococci because
D) it produces DNase
Which selective medium is used for the isolation of Gram-positive bacteria?
A) phenylethyl alcohol agar
A Gram-positive coccus that is catalase-positive, coagulase-negative, and resistant to novobiocin is
C) Staphylococcus saprophyticus
What media is selective for N. gonorrhoeae?
A) MTM and NYC
MRSA is confirmed by an antibiogram indicating resistance to:
A) oxacillin
Identify the organism most likely isolated on this plate.
Staphylococcus aureus
Group A Streptococcus
Enterococcus
Streptococcus pneumoniae
S. pyogenes
An autopsy performed on an 8-year-old child revealed Waterhouse-Friderichsen syndrome. This syndrome is associate with infection of the adrenal glands, leading to uncontrolled bleeding. Blood and throat cultures taken just prior to death were positive for which organism?
C) Neisseria meningitidis
A b-hemolytic Gram-positive coccus was isolated from the cerebrospinal fluid of a 2-day-old infant with symptoms of meningitis. The isolate grew on sheep blood agar under aerobic conditions and was resistant to a Bacitracin disk. What steps would you take to identify this organism?
type the colonies for the different Strep by latex agglutination.
A Gram-positive, b-hemolytic, catalase-positive coccus is coagulase-negative by the slide coagulase test. Which of the folowing is the MOST appropriate action in identification of this organism?
B) do a tube coagulase test to confirm the slide test
The diagnosis of Neisseria gonorrhoeae in females is best made from:
C) an endocervical culture
Viridans streptococci can be differentiated from Streptococcus pneumoniae by:
B) bile solubility test
Gram-positive cocci in chains are seen on a Gram stain from a blood culture. Which media would you select for subculture?
A) sheep's blood agar with an A disc and P disc and bile esculin
Which of the following is the MOST reliable test to differentiate Neisseria lactamica from Neisseria meningitis?
D) lactose fermentation
Which of the following pathogens does NOT require an increased CO2 incubation environment in order to achieve optimal growth?
A Gram-negative coccus that can be described as oxidase-positive, glucose-negative, maltose-negative, sucrose-negative, and lactose negative is
D) Moraxella catarrhalis.
The primary virulence factor of Streptococcus pneumoniae is
A) its polysaccharide capsule
Which of the following could be used as positive and negative controls, respectively, for the PYR test?
D) Enterococcus faecalis and Viridans streptococci
(T/F) The advantage of molecular assays for Neisseria gonorrhoeae is they are very sensitive, they are faster than culturing the organism and the same specimen can be used to detect Chlamydia trachomatis.
True
The most common etiologic agent of infections associated with the surgical insertion of prosthetic devices such as artificial heart valves and cerebrospinal fluid shunts is
B) Staphylococcus epidermidis
The Viridans streptococci are commensals in which areas of the body?
B) All these areas may contain viridans strep.
(T/F) N. gonorrhoeae is particularly sensitive to dry conditions and fluctuations in temperature. Specimens for culture should be kept moist, must not be refrigerated and must be plated as soon as possible after collection.
True
(T/F) Infections caused by MRSA, methicillin-resistant Staphylococcus aureus, were formerly considered nosocomial infections. Now patientsentering the hospital are routinely checked for colonization by MRSA by swabbing the nares for culture.
