M00 - Combo Rotation Exam

  1. An antibiotic used to suppress or kill contaminating fungi in media is:

    B)  amphotericin B
  2. A first morning sputum is received for culture of acid fast bacilli. It is digested and concentrated by the Nacetyl L cysteine alkali method. Two Sabouraud dextrose slants are incubated in the dark at 35°C with 5%10%CO . The smears reveal acid fast bacilli, but the slants show no growth after 8 weeks. The explanation is:

    C)  improper media used
  3. Which of the following reagents is added to detect the production of indole?

    B)  p Dimethylaminobenzaldehyde
  4. Organisms that should be considered in a waterborne outbreak of diarrheal disease include:

    A)  Giardia lamblia and Cryptosporidium parvum
  5. What are the most appropriate screening tests to presumptively differentiate and identify the nonfermentative gram negative bacilli from the Enterobacteraciae?

    C)  Oxidase, TSI, nitrate reduction , growth Correct! on MacConkey agar
  6. A lactose negative, motile, Gram negative rod was isolated from stool. The following tests were inoculated resulting in the following:
    TSI K/
    A H S (trace)
    citrate, urea, VP = ve
    Phenylalanine deaminase and arginine dehydrogenase = ve
    indole, ornithine and lysine decarboxylase = +ve

    Which organism is it likely to be?

    a) Edwardsiella tarda
    b) Salmonella typhi
    c) Proteus mirabilis
    d) Yersinia enterocolitica
    a) Edwardsiella tarda
  7. Routine laboratory testing for Treponema pallidum involves:

    D)  Serological analysis
  8. A Gram positive coccus that is B-hemolytic on sheep blood agar and is frequently associated with meningitis in a newborn is most likely:

    D)  Streptococcus agalactiae
  9. DFA is used for the detection of:

    A)  antigen
  10. HIV may be diagnosed by

    A)  All of the above
  11. Biochemically, the Enterobacteriaceae are gramnegativerods that:

    A)  Ferment glucose, reduce nitrate to nitrite and are oxidase negative.
  12. Kanamycin inhibits bacterial growth by:

    D)  Interfering with protein synthesis
  13. A 23 year old female is seen by her physician for symptoms of uncomplicated cystitis. A culture of her urine grew a Gram positive coccus, which would most likely be

    A)  Staphylococcus saprophyticus
  14. Subacute endocarditis is most frequently associated with

    A)  viridans streptococci
  15. Fecal cultures are inoculated on thiosulfate citrate bile salts sucrose agar specifically for the isolation of

    D)  Vibrio species
  16. Identify the swarming gramnegativebacillus that can be described as

    A)  Proteus mirabilis.
  17. The presence of HbsAg, anti HBc and often Hbe Ag is characteristic of:

    B)  early acute HBV hepatitis
  18. Which of the following are NOT characteristics of group D enterococci:

    A)  CAMP positive
  19. TSI tubes were inoculated with isolates recovered from a stool specimen. They were incubated at 35°C o/n aerobically. Indicate the appropriate interpretation of the tubes shown. Tube "C" is an uninoculated control; NC = no change.

    Image Upload 1

    C)  tube 1 K/NC, tube 2 K/A, tube 3 K/A H S, tube 4 A/A Gas
  20. The method most often used to check for a protective immune response (sufficiently high IgG levels) against most childhood viral diseases is

    C)  EIA
  21. Image Upload 2

    D)  Candida albicans
  22. Which Gram negative bacilli produce black pigment and brick red fluorescence when exposed to an UV light source?

    D)  Prevotella & Porphrymonas
  23. Which of the following could be used for the routine isolation and speciation of yeast?

    D)  CHROM agar
  24. Colonies of Neisseria sp. turn color when a redox dye is applied. The color change is indicative of the activity of the bacterial enzyme

    A)  Cytochrome oxidase
  25. The etiologic agent of whooping cough is:

    D)  Bordetella pertussis
  26. An infant was hospitalized with a severe, tender erythema that started on the face and then spread to the trunk and extremities. The child's epidermis was loose, and large areas of skin could be peeled off, leaving the sensitive underlying dermis exposed. The condition described is most consistent with a clinical syndrome associated with which organism?

    B)  Staphylococcus aureus
  27. "Chinese letters" best describe which of the following seen on Gram stain?

    C)  Corynebacterium diphtheriae
  28. In Complement fixation, what are the expected results from the positive and negative control?

    B)  Positive - no lysis / Negative – lysis
  29. IFA differs from DFA in that:

    B)  Antibodies to the virus of interest are detected
  30. A single codon is made up of how many nucleotides?

    A)  three
  31. The most common etiologic agent of infections associated with the surgical insertion of prosthetic devices such as artificial heart valves and cerebrospinal fluid shunts is

    B)  Staphylococcus epidermidis
  32. Rickettsiae infecting man multiply preferentially within which of the following cells?:

    A)  endothelial
  33. Primary amoebicmeningoencephalitis(PAM) may be caused by:

    A)  Naegleria fowleri
  34. A leg wound culture from a hospitalized 70 year old diabetic man grew motile, lactose negative colonies on MacConkey agar. Given the following biochemical reactions at 24 hours, what is the most probable organism?

    H S (TSI) = ve
    MR = ve
    Phenylalanine deaminase = ve
    Ornithine and lysine decarboxylase = +ve
    Arginine decarboxylase = ve
    Gelatin = +ve
    Indole = ve
    VP = +ve
    DNase = +ve
    Urea = ve

    D)  Serratia marcescens
  35. Flocculation tests for syphilis use antigen composed of:

    A)  cardiolipin
  36. Which combination of tests can presumptively identify Enterohemorrhagic Escherchia coli (EHEC)?

    D)  fermentation on MAC and no fermentation on SMAC
  37. Which of the following characteristics would NOT be used to identify Streptococcus species?

    C)  catalase
  38. IMMUNO vaccination immunoglobulin response

    A)  All of the above
  39. In which technique is the signal amplified instead of the actual target DNA?

    C)  branched DNA amplification
  40. Precipitates of diphtheria toxin and antitoxin formed in agar gels are an in vitro means for detecting toxigenic strains of Corynebacterium diphtheriae. The name of this test procedure is the

    C)  Elek test
  41. The etiologic agent of epidemic relapsing fever is Borrelia recurrentis, which is transmitted by:

    A)  human body lice
  42. Image Upload 3

    A)  Coccidiodes immitis
  43. Most people have antibodies to the following because of previous exposure that caused little or no illness.

    A)  All of the above
  44. Chocolate agar base containing vancomycin, colistin, nystatin, and trimethoprim is also known as___________ and is used for ______________________

    B) modified ThayerMartinagar, cultivation and isolation of Neisseria
  45. The term syncytia is used to describe

    D)  multinucleated cells formed from the fusion between cells penetrated by virions and other host cells nearby.
  46. Which group of tests differentiates Acinetobacter spp. from Pseudomonas aeruginosa?

    C)  Oxidase, motility, growth at 42°C
  47. Chlamydia trachomatis produces all the following conditions in adult women EXCEPT:

    D)  Bacterial vaginosis
  48. The specimen of choice for detecting rotavirus is a

    B)  stool specimen.
  49. Specimens for Mycobacterium studies that require concentration, decontamination, or liquefaction include

    D)  sputum
  50. The image shows a smear from a vaginal swab indicating the presence of small rods and clue cells. This indicates bacterial vaginosis due to:

    C)  Gardernella vaginalis
Card Set
M00 - Combo Rotation Exam
M00 - Combo Rotation Exam