Chapter 9-Controlling Microbial Growth in the Environment Lecture notes

Removal or destruction including bacteria, viruses endospores, etc...
Either in or on an object
What is the Terminology?

Physical or chemical agent (disinfectant) to inhibit or destroy microorganisms on inanimate objects.  What is the terminology?

Which of the following substances or processes kills microorganisms  on laboratory surfaces?
A) degermers
B) antiseptics
C) disinfectants
D) pasteurization

Types of Disinfectants
1)____
2)___
3)___
4)___
5)____

Refers to the removal of microbes from a surface by scrubbing___.
ex: washing hands
alcohol scrub prior to a shot

Environment or procedure is free of contamination

Is the Inhibition/killing of microorganisms on skin or tissue to destroy pathogens

Is the disinfection of plates/utensils in a public setting

Scientists use this suffix to decribe the inhibition of growth metabolism but does not kill microbes.
ex: Refrigerator=bariostatic

Scientists use this suffix to refer to agents that destroy/kill microorganisms.
ex: Bacteriacides
Virucides
Fungicides
Germacides

The action of antimicrobial agents on the 
Cell Wall
Cell Membranes
Protein
Nucleic Acid

This Method dentures proteins
- integrity of cell membrane/cell well
-structure/function of nucleic acids

This method refers to killing all cells in a broth within 10 mins at the Lowest temp

This time it takes to kill all cells in a volume of liquid @ a set temp.

This Heat-related method kills cells by denaturing proteins to sanitize & to pasteurize
H2O is used because its a better conductor of heat

This method kills the vegetative cells of bacteria, but doesn't kill endospores & some viruses

-Steam is heat released
-uses both high temp & ^pressure to prevent steam from escaping ^ boiling temp
What is this heat-related method?

This method of heating foods to kill pathogens in milk, fruit juices
not sterilization

Heat milk to 72°c for 15 sec is called?

For substances such as powders and oils that can't tolerate moist heat & requires higher temperatures for longer times than moist heat
& Incineration is the ultimate means of sterilization

An example of dry heat  is the oven that is set on what temp for how long?

1.)This method of controlling microbes consists of temps of 0°c to 7°c &
2.) this method consisits of temps <0°c
-no liquid H20 or chemical rxns

Microbes that are not affected by refridgeration are___ & ____

Ice crystals have time to form and puncture cell membranes inhibiting microbial growth is called______ ______.

What are the standard size for a filter?

What are the 3 examples of Ionizing Radiation?

This radiation has high energy, good for killing
thick meat
spices
dental/med supplies

This radiation has deeper penetration
-longer time 
-used for meats, spices 
fruits & vegetables} kills the cells and lets them have a higher shelf life

This radiation has the Deepest perpetrators
-longest time
-less energy