Removal or destruction including bacteria, viruses endospores, etc...
Either in or on an object
What is the Terminology?
Physical or chemical agent (disinfectant) to inhibit or destroy microorganisms on inanimate objects. What is the terminology?
Which of the following substances or processes kills microorganisms on laboratory surfaces?
Types of Disinfectants
- UV Light
Refers to the removal of microbes from a surface by scrubbing___.
ex: washing hands
alcohol scrub prior to a shot
Environment or procedure is free of contamination
Is the Inhibition/killing of microorganisms on skin or tissue to destroy pathogens
Is the disinfection of plates/utensils in a public setting
Scientists use this suffix to decribe the inhibition of growth metabolism but does not kill microbes.
Scientists use this suffix to refer to agents that destroy/kill microorganisms.
The action of antimicrobial agents on the
- CW=withstands osmotic pressure if damaged cells burts
- CM= holds in contents of cytoplama(DNA) & it leaks out
- Pro= diff functions-3d shape heat, chem. denatures act on ribozomes b/f prot are made
- Nuc= get altered or destroyed making fatal mutation
This Method dentures proteins
- integrity of cell membrane/cell well
-structure/function of nucleic acids
This method refers to killing all cells in a broth within 10 mins at the Lowest temp
Thermal death point
This time it takes to kill all cells in a volume of liquid @ a set temp.
Thermal Death Time
This Heat-related method kills cells by denaturing proteins to sanitize & to pasteurize
H2O is used because its a better conductor of heat
This method kills the vegetative cells of bacteria, but doesn't kill endospores & some viruses
-Steam is heat released
-uses both high temp & ^pressure to prevent steam from escaping ^ boiling temp
What is this heat-related method?
This method of heating foods to kill pathogens in milk, fruit juices
Heat milk to 72°c for 15 sec is called?
For substances such as powders and oils that can't tolerate moist heat & requires higher temperatures for longer times than moist heat
& Incineration is the ultimate means of sterilization
An example of dry heat is the oven that is set on what temp for how long?
171°c x 1 hr
1.)This method of controlling microbes consists of temps of 0°c to 7°c &
2.) this method consisits of temps <0°c
-no liquid H20 or chemical rxns
Microbes that are not affected by refridgeration are___ & ____
Ice crystals have time to form and puncture cell membranes inhibiting microbial growth is called______ ______.
What are the standard size for a filter?
0.25um - 0.01 um
What are the 3 examples of Ionizing Radiation?
- 1.) Electron beams
- 2.) Gamma Rays
- 3.) XRAYS
This radiation has high energy, good for killing
This radiation has deeper penetration
-used for meats, spices
fruits & vegetables} kills the cells and lets them have a higher shelf life
This radiation has the Deepest perpetrators