Removal or destruction including bacteria, viruses endospores, etc...
Either in or on an object
What is the Terminology?
Sterilization
Physical or chemical agent (disinfectant) to inhibit or destroy microorganisms on inanimate objects. What is the terminology?
Disinfection
Which of the following substances or processes kills microorganisms on laboratory surfaces?
C) disinfectants
Types of Disinfectants
1)____
2)___
3)___
4)___
5)____
UV Light
Heat
Alcohol
Lysol
Bleach
Refers to the removal of microbes from a surface by scrubbing___.
ex: washing hands
alcohol scrub prior to a shot
Degerming
Environment or procedure is free of contamination
Aseptic
Is the Inhibition/killing of microorganisms on skin or tissue to destroy pathogens
Antisepsis
Is the disinfection of plates/utensils in a public setting
Sanitization
Scientists use this suffix to decribe the inhibition of growth metabolism but does not kill microbes.
ex: Refrigerator=bariostatic
stasis/static
Scientists use this suffix to refer to agents that destroy/kill microorganisms.
ex: Bacteriacides
Virucides
Fungicides
Germacides
-cide/-cidal
The action of antimicrobial agents on the
Cell Wall
Cell Membranes
Protein
Nucleic Acid
CW=withstands osmotic pressure if damaged cells burts
CM= holds in contents of cytoplama(DNA) & it leaks out
Pro= diff functions-3d shape heat, chem. denatures act on ribozomes b/f prot are made
Nuc= get altered or destroyed making fatal mutation
This Method dentures proteins
- integrity of cell membrane/cell well
-structure/function of nucleic acids
High Temperature
This method refers to killing all cells in a broth within 10 mins at the Lowest temp
Thermal death point
This time it takes to kill all cells in a volume of liquid @ a set temp.
Thermal Death Time
This Heat-related method kills cells by denaturing proteins to sanitize & to pasteurize
H2O is used because its a better conductor of heat
Moist Heat
This method kills the vegetative cells of bacteria, but doesn't kill endospores & some viruses
Boiling
-Steam is heat released
-uses both high temp & ^pressure to prevent steam from escaping ^ boiling temp
What is this heat-related method?
Autoclaving
This method of heating foods to kill pathogens in milk, fruit juices
not sterilization
Pasteurization
Heat milk to 72°c for 15 sec is called?
Flash Pasteurization
For substances such as powders and oils that can't tolerate moist heat & requires higher temperatures for longer times than moist heat
& Incineration is the ultimate means of sterilization
Dry Heat
An example of dry heat is the oven that is set on what temp for how long?
171°c x 1 hr
1.)This method of controlling microbes consists of temps of 0°c to 7°c &
2.) this method consisits of temps <0°c
-no liquid H20 or chemical rxns
Refridgeration
Freeze
Microbes that are not affected by refridgeration are___ & ____
Yersine
Listeria
Ice crystals have time to form and puncture cell membranes inhibiting microbial growth is called______ ______.
Slow Freezing
What are the standard size for a filter?
0.25um - 0.01 um
What are the 3 examples of Ionizing Radiation?
1.) Electron beams
2.) Gamma Rays
3.) XRAYS
This radiation has high energy, good for killing
thick meat
spices
dental/med supplies
Electron beams
This radiation has deeper penetration
-longer time
-used for meats, spices
fruits & vegetables} kills the cells and lets them have a higher shelf life
Gamma Rays
This radiation has the Deepest perpetrators
-longest time
-less energy
