Microbiology Lab Exercise 8-9

what is the primary stain? what does it stain?

Malachite green; spores
what is the secondary stain? what does it stain?

safranin; cells
used on bacteria that are diff to decolorize. heat melts the wax like material, stain, then cooled seals in the color that the alcohol cannot rinse.

acid fast
what compounds are found in the cell walls of the acid fast bacteria

mycolic acids
what type of cultures work best with acid fast staining?

older cultures because the mycolic acid accumulates slowly
What method was used to conduct the acid-fast stain?

Ziehl-Neelsen method
why is heat necessary for this stain?

heat partially melts the wax to allow the color in
What genus of bacteria exhibit acid fast characteristics?

mycobacterium tuberculosis and mycobacterium leprae
What diseases are associated with this genus?

tuberculosis and leprosy
What is the primary stain? What is the secondary stain?

carbolfuchsin, methylene blue

Author

hey_itsdarra

323711

Card Set

Microbiology Lab Exercise 8-9

Description

exam 1

Updated

2016-09-27T02:45:05Z