Microbiology Lab Exercise 7-8

depending on the amount of secretions produced and their consistency, the secretions are sometimes visible around row bacteria

slime layer
slime layer form a well defined structure around the bacterium

capsule
having a very defined structure as well as a slime layer around a bacteria

glycocalyx
elimination of metabolic wastes and prevention of dehydration (drying)

function of capsule
what factors determine the exact composition of a capsule?

polysaccharides, glycoproteins, polypeptides
which two staining techniques are combined in the capsule stain

direct staining and negative staining
microorganism used in the capsule stain in lab

K. pneumoniae
Is heat fixing used in capsule stain?

no heat fixing in capsule stain
extremely resistant to heat, drying out and many chemicals

endospore
metabolic functions at an almost undetectable level

dormant state
What is the purpose of endospores

to ensure the survival of a bacterium through periods of environmental stress
what is the spore coat made of?

cystine rich keratin like proteins
the genera of bacteria that have endospores

bacillus and clostridium
what type of cultures should be used with endospore staining

24 hr 72 hr
genera of bacteria that have endospores

bacillus magaterium
why is heat used in endospore staining ?

used to drive a stain into the endospore
The names of the two methods used to stain endospores

Schaeffer-Fulton and Dorner method

Author

hey_itsdarra

323710

Card Set

Microbiology Lab Exercise 7-8

Description

exam 1

Updated

2016-09-27T03:14:37Z