Microbiology Lab Exercise 4-5

  1. Is a group of cells which arises from a single cell.
  2. starting new cultures from the isolated colonies
  3. A common method for isolating bacteria from a mixed culture
    streak plate method
  4. what is the purpose of the streak plate method
    isolates pure colonies of bacteria; "thins out" bacterial growth into successive sectors of the Petri until indiv colonies are obtained
  5. Know some possible sources of contamination when transferring bacteria.
    airborne microorganisms (including fungi), microbes picked up from the researcher’s body, the lab bench-top or other surfaces, microbes found in dust, as well as microbes found on unsterilized glassware and equipment,
  6. M. Luteus on a slant
    yellow, individual colonies
  7. M. Rosesus on a slant
    only red bacteria
  8. passing the slide rapidly through the flame of the Bunsen burner...
    Heat fix
  9. where the objective is to stain the bacterium itself... used in direct staining
    direct stain
  10. where the objective is to stain the background, but leave the bacteria itself
    negative stain
  11. a polysaccharide which is initally extracted from the marine red algae. Excellent solid support for growing bacteria; it resists degradation by most bacteria.
  12. A tube in which the agar has been solidified at an angle; provide a good surface for inoculation.
  13. Agar poured into Petri dishes. Not good for long term sto of bacteria, they dry out quicker.
    pour plate
  14. involving a single stain
    simple stain
  15. Methylene blue
    basic stain (direct stain)
  16. crystal violet
    basic stain (direct stain)
  17. Safranin
    basic stain (direct stain)
  18. Congo red
    acidic stain (negative stain)
  19. Nigrosin
    acidic stain (negative stain)
  20. Purpose of heat fixing
    prevent the organisms from being removed from slide during staining procedure
  21. when is it and is not appropriate to heat fix a smear?
    heat fix with a direct stain, not negative staining.
Card Set
Microbiology Lab Exercise 4-5
exam 1