CLS05 - Enterobacteriaceae (Agar)

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  1. SBA agar
    • Sheep's Blood Agar
    • Allows visualization of B hemolysis (some E. coli)
    • Swarming of Proteus
    • Mucoid colonies of Klebsiella pneumoniae
  2. MAC agar
    • MacConkey Agar
    • Detection/isolation of gram- from clinical, dairy, food, water, etc
    • Lactose and Neutral red: differential agents (fermentation)
    • Bile salts and Crystal Violet: inhibit gram+
    • **lactose fermentors (typical enteric) will grow pink, non-fermentors will grow clear
    • Image Upload 1
  3. EMB agar
    • Eosin-methylene Blue Agar
    • For isolation of G- enteric bacteria (looking for coliforms)
    • Eosin Y and Methylene Blue: selective age and differential pH indicator
    • Lactose: differential agent (fermentation)
    • **lactose negative = clear
    • **lactose positive = pinkish/purple
    • **E. coli = metallic green
    • Image Upload 2
  4. HE agar
    • Hektoen Enteric Agar
    • Isolation/cultivation of G- enteric ESPECIALLY Shigella
    • Lactose, saccharose, salicin: differential agents
    • Soldium thiosulfate and Ferric ammonium citrate: for H2S production (black colonies)
    • **E. coli turns medium orange
    • **Salmonella shows "bullseye colonies" w/ black center
    • **Shigella shows no bullseye, agar remains blue
    • Image Upload 3
  5. XLD agar
    • Xylose Lysine Desoxycholate Agar
    • Isolation/differentation of enterics esp. Shigella
    • Lysine, lactose, succharose: fermentable carbs
    • Sodiumthiosulfate and Ferric ammonium citrate: H2S production
    • **Salmonella has bullseye colonies
    • **Shigella appears clear w/ no bullseye, agar remains red
    • **E. coli changes agar yellow
    • Image Upload 4
  6. SS agar
    • Salmonella-Shigella Agar
    • isolation of pathogenic enteric bacilli, esp Salmonella and some Shigella
    • Lactose and neutral red: differential agents
    • sodium thiosulfate, and ferric citrate: H2S detection
    • **Salmonella appears black (H2S)
    • **Shigella appears clear
    • **E. Coli grows pink (like MAC)
    • Image Upload 5
  7. CIN agar
    • Cefsulodin-Irgasan-Novobiocin agar
    • AKA YSA (Yersinia selective agar)
    • For isolation of Yersinia enterocolitica
    • antibiotics inhibit most enterobacteria
    • **Y. enterolytica shows bullseye morphology (red)
    • Image Upload 6
  8. SMAC agar
    • Sorbitol MacConkey Agar
    • For detection of sorbitol-nonfermenting E. coli serotype O157:H7 (assoc w/ hemorrhagic colitis)
    • same as MAC, but sorbitol replaces lactorse
    • **this serotype cannot ferment sorbitol and WILL NOT have a pink color, find the clear colonies!
    • Image Upload 7
  9. Broth cultures
    • Gram neg broth isolates enterics
    • mannitol: inhibits enterics by slowing growth
    • selenite broth and tetrathionate broth: enrich recovery of Salmonella and Shigella (Selenite toxic to most enterics)
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CLS05 - Enterobacteriaceae (Agar)
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CLS05 - Enterobacteriaceae (Agar)
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