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Gardnerella vaginalis - gram stain appearance, site, disease, treatment, diagnostic
- gram: Small, pleomrophic gram-variable or negative coccobacilli and short rods
- diagnostic: clue cell is epithelial cells covered w/ bacteria ("velvet coat")
- site: commensal in human urogenital
- disease: bacterial vaginosis (BV), UTI
- treatment: metronidazole
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Gardnerella vaginalis - media growth/no growth, important morphology
- grows: SBA, chocolate agar, human blood bilayer tween (HBT) agar
- SBA - small pinpoint, non-hemolytic
- HBT - hemolytic
- *COMPARE FOR DIAGNOSTIC
- no growth: MacConkey, blood culture bottles with SPS (sodium polyanetholsulfate)
- 5-10% CO2 during incubation
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Human blood blayer Tween agar - what is it? Why is it used? Important ingredients?
- selective and differential bilayer medium
- For primary isolation and presumptive ID of Gardnerella vaginalis
- Ingredients: columbia agar base
- corn starch - neutralizes fatty acids
- antibiotics - CNA and amphotericin B select for G. vaginilas
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Arcanobacterium - gram stain appearance, diseases
- Delicate, curved, gram + rods w/ pointed ends
- VERY UNCOMMON - pharyngitis, cellulitis
- *NOTE - usually not causative
- **NOTE - will result in a "reverse CAMP test"
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Lactobacillus - gram stain appearance, diseases, drug of choice
- gram: Pleomorphic, occuring in long chaining rods
- disease: Skin commensal, Uncommon cause of bacteremia in immunocompromised
- *NOTE - maintain proper pH balance in vag, we WANT high qty in vag smear
- drug: resistant, but rarely cause infection
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Erysipelothrix - gram stain appearance, diseases, drug of choice
- gram: Short rods and long filaments
- disease: Erysipeloid skin lesions
- Mostly zoonotic infections from abrasion or puncture wound
- drug: Penicillin, cephalosporin, erythromycin, tetracyline, ciprofloxacin
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