Biology 3400 - Lecture 8

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  1. Virion
    Extracellular form of a virus
  2. Naked virus
    Capsid enclosed DNA, but no envelope
  3. Virus classification by host
    Bacterial, Archael, Animal, Plant, Other
  4. Virion structure
    • Capsid - Protein shell around genome
    • Capsomere - Capsid subunit
    • Nucleocapsid - Nucleic acid packaged in virion
  5. Viral envelope
    Lipid bilyayer with associated proteins
  6. Neuraminidases
    Enzyme cleaves glycosidic bonds
  7. Titer
    Number of infectious units per volume of fluid
  8. Permissive cell
    Cell allowing complete replication cycle of a virus
  9. CRISPR
    • Similar to RNA interference
    • Sequence specific
    • Prokaryotes
  10. 5-hydroxymethulcytosine
    DNA modification used by T4 to prevent restriction
  11. Viral life cycle modes
    • Virulent - Cell lysis
    • Temperate - Formation of prophage
  12. Lysogen; Lysogeny
    • Lysogen - Bacteria containing a prophage
    • Lysogeny - Dormant prophage is replictated but not expressed
  13. cos sites
    • 12bp sticky ends
    • Circular after penetration
    • Can integrate into host DNA
  14. Rolling circle replication
    Lambda genome replication (lytic)
  15. Lytic mode repressor proteins
    cl protein, Cro
  16. Bacterial viruses
    • dsDNA
    • Naked (occasionally lipid env.)
  17. Animal viruses
    • Entire virion enters cell
    • More viruses have envelope (from host)
  18. Types of animal cell infection
    • Persistent - Slow release of virus (no lysis)
    • Latent - Present, but no replication (can revert to lytic)
  19. Retrovirus key genes
    • gag - encode structural proteins
    • pol - encode reverse transcriptase
  20. Viral metagenome
    Total of all viral genomes in an environment
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Biology 3400 - Lecture 8
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Biology
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