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Biology 3400 - Lecture 8
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Virion
Extracellular form of a virus
Naked virus
Capsid enclosed DNA, but no envelope
Virus classification by host
Bacterial, Archael, Animal, Plant, Other
Virion structure
Capsid - Protein shell around genome
Capsomere - Capsid subunit
Nucleocapsid - Nucleic acid packaged in virion
Viral envelope
Lipid bilyayer with associated proteins
Neuraminidases
Enzyme cleaves glycosidic bonds
Titer
Number of infectious units per volume of fluid
Permissive cell
Cell allowing complete replication cycle of a virus
CRISPR
Similar to RNA interference
Sequence specific
Prokaryotes
5-hydroxymethulcytosine
DNA
modification
used by T4 to prevent
restriction
Viral life cycle modes
Virulent - Cell lysis
Temperate - Formation of prophage
Lysogen; Lysogeny
Lysogen - Bacteria containing a prophage
Lysogeny - Dormant prophage is replictated but not expressed
cos
sites
12bp sticky ends
Circular after penetration
Can integrate into host DNA
Rolling circle replication
Lambda genome replication (lytic)
Lytic mode repressor proteins
cl protein, Cro
Bacterial viruses
dsDNA
Naked (occasionally lipid env.)
Animal viruses
Entire virion enters cell
More viruses have envelope (from host)
Types of animal cell infection
Persistent - Slow release of virus (no lysis)
Latent - Present, but no replication (can revert to lytic)
Retrovirus key genes
gag - encode structural proteins
pol - encode reverse transcriptase
Viral metagenome
Total of all viral genomes in an environment
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Author
Ant
ID
317127
Card Set
Biology 3400 - Lecture 8
Description
Biology
Updated
2016-03-10T02:50:04Z
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