Molec Cell Midterm 1

• alanine
• valine
• isoleucine
• leucine
• methionine
• phenylalanine
• tyrosine
• tytophan

to solubilize membrane proteins

2 fatty acid chains attached to 2 carbons in a glycerol molecule, 3rd carbon attaches to a phosphate group which is attached to the polar head

• micelles - one layer, hydrophobic tails pointing in
• liposomes - bilayered, there is a core inside

• Fluorescence Recovery After Photobleaching (FRAP)
• fluorescent proteins are spread out evenly throughout the membrane
• shine laser on membrane to bleach it so it doesn't fluoresce anymore
• recover fluorescence by moving around proteins = fluidity

• temperature - more heat = more fluid
• cholesterol - more cholesterol = less fluid

• sheet-like structures (lipid leaflets)
• contain mostly lipids & proteins
• both proteins & lipids mediate function
• non-covalent assemblies
• fluid
• closed boundaries
• asymmetric (cytosolic face and exosplasmic face)

• glycerol
• fatty acids
• phosphate group
• alcohol head group

• saturated - straight 
• unsaturated - bent
• saturated fatty acids have higher stability but less fluidity

• stiffens the membrane
• extremely hydrophobic

• lipid-anchor protein
• peripheral membrane protein
• integral membrane protein

tether proteins to the exoplasmic face