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Exam 2 Flashcards - Set 22
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Name the molecular technique:
- ultracentrifugation and selective extraction with detergents
Cell Fractionation
Name the molecular technique:
- makes it possible to fix the position of individual atoms in a molecule
X-ray diffraction (crystallography)
Name the molecular technique:
- Study molecules in solution (native environment)
NMR
Constitute all of the cell's mRNA molecules
transcriptome
Constitute all of a cell's proteins
proteome
Methods used to study transcriptome
Transcriptomics
Methods used to study proteome
Proteomics
The transcriptome and proteome ____ change in response to a changing environment.
continually
Name two methods of high throughput gene expression analysis:
1. DNA microarays
2. RNA seq
What enzyme do both, DNA microarrays and RNA seq use?
Reverse transcriptase
Name the type of analysis:
DNA microarray
Name the technique:
- used to view and capture fluctuations in the proteome
Two-dimensional gel electrophoresis
What does the first dimension of two-dimensional gel electorphoresis do?
Sepearates proteins by their charge (isoelectric focusing)
What does the second dimension of two-dimensional gel electrophoresis do?
Further separates proteins by their molecular weights
Name the technique:
Two-dimensional gel electrophoresis
How are proteins identified?
Must be broken down into individual peptides
Author
tulipyoursweety
ID
316969
Card Set
Exam 2 Flashcards - Set 22
Description
Experimental (Micro)biology and Biotechnology I
Updated
2016-03-07T19:19:57Z
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