Archaea resemble eukaryotes in all of the following EXCEPT:
A) nuclear membrane
The primer in DNA replication is _____ starter sequence with a free _____ group.
A) an RNA; 3' OH
If the sequence of one strand of DNA is 5' TCGATC 3', what is the sequence of the complementary strand?
C) 5' GATCGA 3'
____ _____ is a complex enzyme that carries out transcription by making RNA copies (called ____) of a DNA template strand.
RNA polymerase, transcripts
In bacteria, the RNA pol holoenzyme if composed of:
- Core polymerase: α2, β, β' which are required for the _____ phase
- ____ factor: σ, which is required for the ____ phase
- elongation
- sigma, initiation
The sigma factor helps the core enzyme detect the ____, which signals the beginning of the gene
promoter
During the initiation of transcription, the first ribonucleoside triphosphate (rNTP) of the new RNA chain is usually a _____.
purine (A or G)
Transcription initiation: _______ binds to the promoter. This is followed by melting of the helix and synthesis of the first nucleotide of the RNA
RNA pol holoenzyme
RNA polymerase synehtesizes RNA at ~ ____ bases/second.
45
Name the bacterial gene transcription termination signal:
- relies on a protein called ___ and a strong pause site at the 3' end of the gene
Rho-dependent, Rho
Name the bacterial gene transcription termination signal:
- Requires a GC-rich region of RNA, as well as 4-8 consecutive U residues
Rho-independent
Name the bacterial gene transcription termination signal:
Rho-dependent
Name the bacterial gene transcription termination signal:
Rho-independent
Name the RNA class:
- encodes proteins
Messenger RNA (mRNA)
Name the RNA class:
- forms ribosomes
Ribosomal RNA (rRNA)
Name the RNA class:
- shuttles amino acids
Transfer RNA (tRNA)
Name the RNA class:
- regulates transcription or translation
Small RNA (sRNA)
Name the RNA class:
- frees ribosomes stuck on damaged mRNA
tmRNA
Name the RNA class:
- carries out enzymatic reactions
Calatytic RNA
The average half-life for mRNA is ____ minutes.
1-3
Genes that are _____ likely evolved from a common ancestral gene.
homologous
______ genes are duplicated via appearance of a new species and have identical function in different organisms.
orthologous
_____ genes are duplicated within a species and have slightly different tasks in a cell.