Exam 2 Flashcards - Set 13

  1. Replication of cellular DNA in most cases is _____, meaning that each daughter cell receives one parental strand and one newly synthesized strand.
    semiconservative
  2. - Replication in bacteria begins at a single origin called _____.
    - Replication forks move in opposite directions around the chromosome.
    - Replication ends at a defined termination (___) sites located opposite to the origin
    • - oriC
    • - ter
  3. True/false: A partially replicated chromosome cannot start new rounds of replication at the two daughter origins before the first round is complete.
    False, A partially replicated chromosome can start new rounds of replication at the two daughter origins before the first round is complete.
  4. Name the major protein involved in DNA replication:
    - initiator protein
    - starts when the ratio of DNA is high to ori sites
    DnaA
  5. Name the major protein involved in DNA replication:
    - helicase
    - takes DNA apart
    DnaB
  6. Name the major protein involved in DNA replication:
    - synthesis of RNA primer
    - provides 3' hydrolyl group
    DNA primase
  7. Name the major protein involved in DNA replication:
    - major replication enzyme
    - needs primer
    DNA Pol III
  8. Name the major protein involved in DNA replication:
    - replaces RNA primer with DNA
    DNA Pol I
  9. Name the major protein involved in DNA replication:
    - relieves DNA supercoiling
    DNA gyrase
  10. A ____ ____ protein (the beta subunit) tethers DNA polymerase to the DNA.
    - without it, DNA pol would frequently "fall off" the DNA molecule
    sliding clamp
  11. The cell coordinates the activity of two DNA Pol III enzymes in one complex. These two enzymes, together with the DNA primase and helicase, form the ______.
    replisome
  12. - To remove RNA primers, cells use _____.
    - ______ enzyme then synthesizes a DNA patch using the 3' OH end of the preexisting DNA fragment as a priming site
    - Finally, _____ repairs the phosphodiester nick using energy from NAD (in bacteria) or ATP (in eukaryotes)
    • - RNase H
    • - DNA Pol I
    • - DNA ligase
  13. A protein called Tus (____ ___ ____) binds to the terminotor sequences (ter) and acts as a counter helicase
    terminus utilization substance
  14. Ringed _____ formed at the completion of replication are separated by topoisomerase IV and the proteins XerC and XerD.
    catenanes
  15. ______ _____ cleave DNA at specific recognition sites, which are usually 4 to 6 bp and _______.
    - May generate blunt of staggered ends
    Restriction endonucleases, plaindromes
  16. _____ ____ ______ can be used to analyze the DNA fragments obtained by treatment with restriction enzymes.
    Agarose gel electrophoresis
  17. The _____ _____ ____ (____) can produce over a million-fold amplification of target DNA within a few hours.
    polymerase chian reaction (PCR)
  18. ______ ______ containing all the genes in an organism are routinely made today.
    Genome libraries (also called clone libraries or clone pools)
  19. ____ ____ allows the study of eukarytoic proteins in prokaryotic cells.
    shuttle vector
  20. DNA replication is divided into three phases:
    1. Initiation: occurs at the origin (_____)
    2. Elongation: occurs at the ______ _____
    3. Termination: occurs at the terminus (___)
    • - oriC
    • - replication forks
    • - ter
Author
tulipyoursweety
ID
316902
Card Set
Exam 2 Flashcards - Set 13
Description
Mechanism of Replication and Methods of DNA Manipulation
Updated