DNA Replication Enzymes

opens the double helix at replication forks by disrupting the hydrogen bonds that hold the two strands together

binds to single strands of DNA and prevents the helix from reforming before it can be used as a template for replication

breaks one or both DNA strands, preventing excessive coiling during replication, and then rejoins them in a more relaxed configuration

links nucleotide subunits to form a new DNA strand from a DNA template

synthesizes short RNA primers on the lagging strand; begins replication of the leading strand

links Okazaki fragments by joining the 3' end of the new DNA fragment to the 5' end of the adjoining DNA