DNA Replication Enzymes

  1. helicase
    opens the double helix at replication forks by disrupting the hydrogen bonds that hold the two strands together
  2. single-strand bonding protein (SSB)
    binds to single strands of DNA and prevents the helix from reforming before it can be used as a template for replication
  3. topoisomerase
    breaks one or both DNA strands, preventing excessive coiling during replication, and then rejoins them in a more relaxed configuration
  4. DNA polymerase
    links nucleotide subunits to form a new DNA strand from a DNA template
  5. DNA primase
    synthesizes short RNA primers on the lagging strand; begins replication of the leading strand
  6. DNA ligase
    links Okazaki fragments by joining the 3' end of the new DNA fragment to the 5' end of the adjoining DNA
Card Set
DNA Replication Enzymes