Biochem 3300 - Metabolism III PII

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  1. Tautomer
    • Molecules with the same molecular formula but different connectivity
    • Interconvert with rapid equillibrium
  2. Hydrolysis of PEP
    • 1. Inorganic phosphate is hydrolyzed
    • 2. The remaining enol is tautomerized into a ketone.
  3. Why does PEP tautomerize?
    Because a ketone is more stable than a terminal alkene.
  4. How is 1,3-Bisphosphoglycerate stabillized after hydrolysis?
    Resonance from ionization of the carbonyl
  5. How is phosphocreatine hydrolysed?
    Covalently with a Schiff base
  6. Structure of coenzyme A?
    ADP-3'-phosphate {phosphate ester linkage} pantothenate (vitamin B) {amide linkage} β-mercaptoethylamine
  7. Functional group of coenzyme A?
    The thiol of β-mercaptoethylamine
  8. What links β-mercaptoethylamine to acetyl?
    A thioester linkage
  9. Oxygen ester -> Thioester

    Will ΔG be pos or neg?
    positive (thioester has more energy)
  10. List from highest energy (ΔG'°) to lowest

    Glucose, Pi, Creatine, 1,3-BisPg, ATP, Glycerol, PEP
    PEP, 1,3-BisPg, Creatine, ATP, Glycerol, Glucose, Pi
  11. Why is ATP so important?
    It has a middle energy, so can act as an intermediate for coupling
  12. What is a group transfer (ATP)?
    A transfer of energy by transferring Pto another compound by energy coupling
  13. Why is AMP a good transient signal?
    It's strained (cyclic) phosphate group is very readily hydrolyzed, meaning it is only transiently phosphorylated
  14. cAMP full name
    3', 5'-cyclic AMP
  15. What are good energy transfer or storage molecules?
    ATP, PPi, polyphosphate, phosphocreatine
  16. What are good group transfer molecules?
    ATP, Coenzyme A
  17. Why is kinetic stability important?
    ATP hydrolysis is spontaneous but kinetically restricted. This allows ATP to serve important metabolic roles.
  18. Equation for redox potential?
    E = E° + [(RT/(nF)]⋅ln[(el. donor)/(el. acceptor)]
  19. Free energy equation for redox
    ΔG = -nFΔE
  20. How are electrons generally transferred?
    Hydrides. (H+ + 2e- + NAD+ -> NADH)
  21. What does NAD stand for?
    Nicotinamide adenine dinucleotide
  22. What is the nicotinamide ring synthesized from?
    Niacin from tryptophan
  23. What is pellagra caused by?
    Niacin/tryptophan deficiency
Card Set
Biochem 3300 - Metabolism III PII
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