bi0 130 chp 20

  1. THis means sthe use of laboratory techniques to isolate and manipulate fragments on DNA.
    Recombinant DNA Technology
  2. THis DNA contains DNA from two or more sources.
    Recombinant DNA
  3. Once inside a host cell, recombinant molecules are replicated to produce?
    identical copies or clones
  4. Why do we copy genes?
    For study/manipulation and to obtain large amounts of product.
  5. In this step of DNA cloning vector DNA is a carrier for the DNA segemtn to be cloned. The Vector makes many copies.
    Step 1
  6. Common vectors are?
    plasmid or viral
  7. This is a carrier of the gene.
  8. In this step of gene cloning chromosomal DNA is put into vector. Cut DNA using restriction enzymes. DNA ligase links DNA.
    Step 2
  9. In this stage od dna cloning the goal is to have recombinant vector taken up. vector also carries a marker. Usually antibiotic resistance. Placed on plate with antibiotics. LacZ gene to eliminate recircularized empty vectors.
    Step 3
  10. Whats another name for restriction enzymes?
    Restriction endonucleases
  11. What is the most common gene for selective marking do we use?
  12. A collection of many recombinant vectors each with a fragment of chromosomal dna.
    DNA library
  13. What are the two types of DNA libraries?
    Genomic Library and cDNA library
  14. These are inserts derived from chromosomal DNA.
    Genomic Library
  15. these use reverse transcriptase to make complementary DNA (cDNA) from mRNA. They lack introns and are simpilar to use.
    cDNA library
  16. This is technique used to separate macromolecules on a gel. Can be use to separate DNA ro proteins.
  17. How does electrophoresis separate DNA?
    By charge, size/length, and mass.
  18. This allows us to amplify DNA.
  19. What are the ingredients for PCR.
    Primers, dNTPs, Taq polymerase
  20. This is a heat stable form of DNA polymerase.
    TaQ polymerase.
  21. What are the cycles of PCR?
    Denaturation, annealing, and synthesis.
  22. After 30 cycles of amplification, a DNA sample will increase
    2E30 fold
  23. The molecular analysis of the entire genome (all the DNA) of a species.
  24. What are the two phases of genomics?
    Mapping of genome and functional genomics
  25. Which genes turn on or off in particular cells.
    Expression analysis
  26. what is BAC and YAC?
    Bacterial or Yeast artificial chromosomes.
  27. This is for contigous. A series of clones that contain overlapping pieces of chromosomal DNA.
  28. This is a method of dna sequencing that uses Dideoxynucleoside triphosphates (ddNTPs) are missing the 3’–OH group and cause chain to terminate. 4 tubes with copies of the single stranded DNA, each with a different labelled nucleotide. DNA polymerase will make complementary strand until ddNTP inserted and chain terminates. After electrophoresis, DNA sequence can be read by determining which base is at the end of the DNA strand. Procedure has been automated using fluorescently labeled ddNTPs all in one tube
    Dideoxy chain-termination method
  29. This can identifu which genes are transcribed by a cell/
  30. Monitors expression of thousand of genes. short sequences of known genes attached to spots on slide. Goal is to find out which genes are transcribed into mRNA.
    DNA microarray or gene chip
  31. This is an organism that carries genes introduced using molecular techniques such as gene cloning.
    Transgenic orgamism
  32. Cloned gene recombines with normal gene on a chromosome.
    Gene replacement
  33. Cloned gene is a mutation that inactivated function and homozygote will not have a gene function.
    Gene knockout.
  34. Production of medically important proteins in livestock mammary glands. Certain proteins more likely to function when expressed in mammals
    molecular pharming
  35. In molecular pharming where is the vector injected?
    in the oocyte
Card Set
bi0 130 chp 20
Chapter 20